解磷菌发酵及溶磷条件的研究

doi:10.13560/j.cnki.biotech.bull.1985.2018-0733

摘要/Abstract

摘要： 从新疆盐碱土中分离纯化得到一株高效解磷菌PS-3,为改善盐碱地磷素供应提供菌种资源。采用单因素和正交试验,对菌株PS-3的发酵条件和溶磷能力进行分析。结果表明,菌株PS-3较适发酵条件为温度35℃、pH 7和盐浓度1%,其对碳、氮源的利用顺序依次为葡萄糖>麦芽糖>果糖>蔗糖>乳糖,硝酸铵>硝酸钠>氯化铵>硫酸铵>尿素,菌株PS-3最适溶磷条件为2%葡萄糖、0.01%硝酸铵、温度35℃、初始pH 7.5、盐浓度2 g/L、接种量4%,在此条件下,其对磷酸三钙的溶解量可达100 2.95 mg/L。该菌株具有一定耐盐性和良好的溶磷能力,在生物溶磷方面具有较好的应用潜力。

关键词: 解磷菌, 发酵条件, 溶磷能力

Abstract: The aim of the present work is to isolate a highly effective phosphate-solubilizing strain PS-3 for improving phosphorus supply of saline-alkali soil in Xinjiang. The single factor experiment and the orthogonal experiment were used to analyze the fermentation conditions and the phosphate-dissolving capability of strain PS-3. The results showed that the appropriate fermentation conditions of the strain PS-3 were temperature 35℃,pH 7 and salt concentration 1%. The order of the strain utilizing carbon and nitrogen was glucose > maltose>fructose>sucrose>lactose,and ammonium nitrate>sodium nitrate>ammonium chloride>ammonium sulfate>urea,respectively. The optimal conditions for the solubilization of strain PS-3 were 2% glucose,0.01% ammonium nitrate,temperature 35℃,initial pH 7.5,salt content 2 g/L,and inoculation amount 4%. The phosphate-solubilizing capability reached the level of 1 002.95 mg/L under this condition. The strain PS-3 is tolerant to salt stress and has desired phosphate-solubilizing capacity,and thus has a better potential for microbial inoculants with phosphorus-solubilizing ability.

Key words: phosphate-solubilizing bacterium, fermentation condition, phosphate-solubilizing capability

韩蕾, 杨乐, 唐金铭, 张雅倩. 解磷菌发酵及溶磷条件的研究[J]. 生物技术通报, 2019, 35(1): 98-104.

HAN Lei, YANG Le, TANG Jin-ming, ZHANG Ya-qian. Fermentation Condition and Phosphate-dissolving Capacity of Phosphate-solubilizing Bacterium[J]. Biotechnology Bulletin, 2019, 35(1): 98-104.

参考文献

[1] Gyaneshwar P, Kumar GN, Parekh LJ, et al.Role of soil microorganisms in improving P nutrition of plants[J]. Plant & Soil, 2003, 245(1):83-93.
[2] Srinivasan R, Alagawadi AR, Yandigeri MS, et al.Characterization of phosphate-solubilizing microorganisms from salt-affected soils of India and their effect on growth of sorghum plants[Sorghum bicolor(L.)Moench][J]. Annals of Microbiology, 2012, 62(1):93-105.
[3] Saurabh Jyoti S, Satinder Kaur B, Yann LB, et al.Potential application of biohydrogen production liquid waste as phosphate solubilizing agent-a study using soybean Plants[J]. Appl Biochem Biotechnol, 2016, 178:865-875.
[4] 鲁如坤, 时正元, 顾益初. 土壤积累态磷研究II.一次大量施磷的产量效应[J]. 土壤, 1995, 27(6):286-289.
[5] Stevenson FJ, Cole MA.Cycles of soils:carbon, nitrogen, phosphorus, sulfur, micronutrients[M]. Wiley:Quarterly Review of Biology, 1999.
[6] 银婷婷, 王敬敬, 柳影, 等. 高效解磷菌的筛选及其促生机制的初步研究[J]. 生物技术通报, 2015, 31(12):234-242.
[7] Swaby RJ, Sperber J.Phosphate dissolving micro-organisms in the rhizosphere of legumes[J]. Nutrition of the Legumes, 1958:289-294.
[8] Ochoaloza FJ, Artiola JF, Maier RM.Stability constants for the complexation of various metals with a rhamnolipid biosurfactant[J]. Journal of Environmental Quality, 2001, 30(2):479.
[9] Yi YHW, Ge Y.Exopolysaccharide:a novel important factor in the microbial dissolution of tricalcium phosphate[J]. World J Microbiol Biotechnol, 2008, 24(7):1059-1065.
[10] 赵小蓉, 林启美. 微生物解磷的研究进展[J]. 中国土壤与肥料, 2001(3):7-11.
[11] Chen YP, Rekha PD, Arun AB, et al.Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities[J]. Applied Soil Ecology, 2006, 34(1):33-41.
[12] Kundu BS, Nehra K, Yadav R, et al.Biodiversity of phosphate solubilizing bacteria in rhizosphere of chickpea, mustard and wheat grown in different regions of Haryana[J]. Indian Journal of Microbiology, 2009, 49(2):120-127.
[13] Nautiyal CS.An efficient microbiological growth medium for screening phosphate solubilizing microorganisms[J]. Fems Microbiology Letters, 1999, 170(1):265-270.
[14] 鲍士旦. 土壤农化分析. [M]. 第3版. 北京:中国农业出版社, 2000.
[15] Bianco C, Defez R.Improvement of phosphate solubilization and Medicago plant yield by an indole-3-acetic acid-overproducing strain of Sinorhizobium meliloti[J]. Applied & Environmental Microbiology, 2010, 76(14):4626.
[16] Mehta P, Walia A, Chauhan A, et al.Plant growth promoting traits of phosphate-solubilizing rhizobacteria isolated from apple trees in Trans Himalayan region of Himachal Pradesh[J]. Archives of Microbiology, 2013, 195(5):357-369.
[17] Bhagwanjee S, Scribante J, Paruk F.Application of potential phosphate-solubilizing bacteria and organic acids on phosphate solubilization from phosphate rock in aerobic rice[J]. The Scientific World Journal, 2013,(2013-10-8), 2013, 2013(4):272409.
[18] Khan MS, Zaidi A, Wani PA.Role of phosphate-solubilizing microorganisms in sustainable agriculture—A review[J]. Agronomy for Sustainable Development, 2007, 27(1):29-43.
[19] 江红梅, 殷中伟, 史发超, 等. 一株耐盐日本曲霉的筛选及其溶磷促生作用[J]. 微生物学报, 2018, 58(5):862-881.
[20] 向文良, 冯玮, 郭建华, 等. 一株解磷中度嗜盐菌的分离鉴定及解磷特性分析[J]. 微生物学通报, 2009, 36(3):320-327.
[21] 胡山, 牛世全, 龙洋, 等. 河西走廊盐碱土壤中一株高效溶磷菌的鉴定及条件优化[J]. 微生物学通报, 2017, 44(2):358-365.
[22] Ahuja A, Ghosh SB, D’Souza SF. Isolation of a starch utilizing, phosphate solubilizing fungus on buffered medium and its characterization[J]. Bioresource Technology, 2007, 98(17):3408-3411.
[23] Jain R, Saxena J, Sharma V.Effect of phosphate-solubilizing fungi Aspergillus awamori S29 on mungbean(Vigna radiata cv. RMG 492)growth[J]. Folia Microbiologica, 2012, 57(6):533-541.
[24] 卫星, 徐鲁荣, 张丹, 等. 一株耐硝酸盐的巨大芽孢杆菌溶磷特性研究[J]. 环境科学学报, 2015, 35(7):2052-2058.
[25] 黄达明, 李倩, 管国强, 等. 一株解磷细菌的筛选、鉴定及其溶磷培养条件的优化[J]. 生物技术通报, 2015, 31(2):173-178.
[26] 李海云, 牛世全, 孔维宝, 等. 猪粪堆肥中一株溶磷菌的筛选鉴定及溶磷能力初步测定[J]. 环境科学学报, 2015, 35(5):1464-1470.