基于石墨烯和功能核酸的Ag+荧光检测方法

doi:10.13560/j.cnki.biotech.bull.1985.2019-1248

生物技术通报 ›› 2020, Vol. 36 ›› Issue (8): 210-216.doi: 10.13560/j.cnki.biotech.bull.1985.2019-1248

基于石墨烯和功能核酸的Ag⁺荧光检测方法

吴雪琪^1,2, 张立佩², 曹阳², 文晓刚³, 周小红²

1.上海师范大学环境与地理科学学院,上海 200234;
2.清华大学环境学院环境模拟与污染控制国家重点实验室,北京 100084;
3.嘉兴学院南湖学院,嘉兴 314001

收稿日期:2019-12-20 出版日期:2020-08-26 发布日期:2020-08-27
作者简介:吴雪琪,男,硕士,研究方向：生物传感;E-mail：15806295142@163.com

Fluorescent Detection of Ag⁺ Using Graphene and Functional Nucleic Acid

WU Xue-qi^1,2, ZHANG Li-pei², CAO Yang², WEN Xiao-gang³, ZHOU Xiao-hong²

1. School of Environmental and Geographic Sciences,Shanghai Normal University,Shanghai 200234;
2. State Key Joint Laboratory of Environmental Simulation and Pollutants Control,School of Environment,Tsinghua University,Beijing 100084;
2. Nanhu College,Jiaxing University,Jiaxing 314001

Received:2019-12-20 Published:2020-08-26 Online:2020-08-27

摘要/Abstract

摘要： 基于核酸适配体错配结合Ag⁺的原理,利用石墨烯对单链DNA和双链DNA结合力的不同,设计开发了C-Ag⁺-C和石墨烯结合的新型荧光传感器,并建立了一种高灵敏度、快速、便捷的荧光检测方法,实现了turn on形式的Ag⁺检测。在优化条件下,检测Ag⁺的线性范围为150-400 nmol/L,检出限为23 nmol/L。该方法在饮用水的实验样品中Ag⁺加标检测的回收率达到96.6%,具备用于实际样品分析的能力。

关键词: 银离子, 碱基错配, 石墨烯, 荧光检测

Abstract: Based on the principle of mismatched binding Ag⁺ of nucleic acid aptamer,using the different binding forces of graphene to single-stranded DNA and double-stranded DNA,a new fluorescent sensor with C-Ag⁺-C and graphene binding was designed and developed. A high sensitivity,fast and convenient fluorescence detection method was developed to achieve the Ag⁺ detection in turn on form. Under the optimization of detection conditions,the turn on fluorescent aptasensor offered a linear detection range of 150-400 nmol/L with a detection limit of 23 nmol/L for Ag⁺. The recovery rate of the method was 96.6% for Ag⁺-labeled detection in the experimental samples of drinking water,showing the ability to be used for actual sample analysis.

Key words: Ag⁺, base mismatch, graphene, fluorescence detection

吴雪琪, 张立佩, 曹阳, 文晓刚, 周小红. 基于石墨烯和功能核酸的Ag⁺荧光检测方法[J]. 生物技术通报, 2020, 36(8): 210-216.

WU Xue-qi, ZHANG Li-pei, CAO Yang, WEN Xiao-gang, ZHOU Xiao-hong. Fluorescent Detection of Ag⁺ Using Graphene and Functional Nucleic Acid[J]. Biotechnology Bulletin, 2020, 36(8): 210-216.

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基于石墨烯和功能核酸的Ag⁺荧光检测方法

Fluorescent Detection of Ag⁺ Using Graphene and Functional Nucleic Acid

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基于石墨烯和功能核酸的Ag+荧光检测方法

Fluorescent Detection of Ag+ Using Graphene and Functional Nucleic Acid

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基于石墨烯和功能核酸的Ag⁺荧光检测方法

Fluorescent Detection of Ag⁺ Using Graphene and Functional Nucleic Acid