生物技术通报 ›› 2021, Vol. 37 ›› Issue (7): 164-174.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0408

• 研究报告 • 上一篇    下一篇

植物源与微生物源生物制剂复配防治根结线虫病

舒洁1,2(), 张仁军1,2, 梁应冲1,2, 陈雅琼3(), 张娟4, 郭建5, 陈穗云2,6()   

  1. 1.云南大学生命科学学院,昆明 650091
    2.云南省作物病害生物防控技术研究中心,昆明 650091
    3.云南省烟草公司昆明市公司,昆明 650051
    4.峨山彝族自治县化念镇农业农村综合服务中心,玉溪 653202
    5.云南省烟草公司红河州公司,弥勒 652399
    6.云南大学生态与环境学院,昆明 650091
  • 收稿日期:2021-04-01 出版日期:2021-07-26 发布日期:2021-08-13
  • 作者简介:舒洁,女,硕士研究生,研究方向:植物病虫害;E-mail: 1043317148@qq.com
  • 基金资助:
    中国烟草总公司云南省公司科技计划项目(2021530000241031);云南省2021年教育厅科学研究基金研究生类项目(2021Y043)

Control of Root-knot Nematode Disease by Compounding Biological Agents from Plant and Microorganisms

SHU Jie1,2(), ZHANG Ren-jun1,2, LIANG Ying-chong1,2, CHEN Ya-qiong3(), ZHANG Juan4, GUO Jian5, CHEN Sui-yun2,6()   

  1. 1. School of Life Sciences,Yunnan University,Kunming 650091
    2. Biocontrol Engineering Research Center of Plant Disease and Pest,Yunnan Province,Kunming 650091
    3. Yunnan Tobacco Company Kunming Branch,Kunming 650051
    4. Huanian Agricultural and Rural Comprehensive Service Center,Yunnan Province,Yuxi 653202
    5. Yunnan Tobacco Company Honghe Branch,Mile 652399
    6. College of Ecology and Environmental Sciences,Yunnan University,Kunming 650091
  • Received:2021-04-01 Published:2021-07-26 Online:2021-08-13

摘要:

番茄根结线虫病严重影响农业生产,利用植物源和微生物源两种不同的生物制剂复配防治番茄根结线虫病,试图探索出适应绿色农业生产需求的一种新的可能性。通过稀释涂布平板法从土壤分离出3株12 h内对根结线虫二龄幼虫(second-stage juveniles,J2)抑杀率分别达89.23%、87.95%、88.97%的生防菌株SJ5、SJ17、SJ19,经16S rDNA扩增测序鉴定分别为耐冷假单胞菌(Pseudomonas psychrotolerans)、黏金黄杆菌(Chryseobacterium gleum)和枯草芽孢杆菌(Bacillus subtilis)。通过平板抑菌圈法证明3种生防菌之间、生防菌与苦参印楝素之间无拮抗现象,适宜复配。盆栽实验初步验证,发酵48 h的3种生防菌发酵液(有效活菌数≥3.0 亿个/mL)与苦参印楝素稀释液(1 000 倍)复配制剂处理组对根结线虫病的防效达67.75%,与化学农药(氟吡菌酰胺、噻唑膦)处理组相比防效无显著性差异,且番茄株高、茎围、苗和根部的生物量均显著性增加,盆栽试验结论初步发现复配方案具有防治番茄根结线虫病害应用潜力。

关键词: 番茄, 根结线虫, 生物防治, 复配

Abstract:

Tomato root-knot nematode disease seriously affects agricultural production. The compound of two biological agents from plant origin and microbial source are used to control tomato root-knot nematode disease,trying to explore a new feasibility to meet the need of green agricultural production. Three strains(SJ5,SJ17 and SJ19)of killing second-stage juveniles(J2)of root knot nematodes were isolated from the soil by the dilution coating plate method with an inhibitory rate of 89.23%,87.95%,and 88.97%,respectively,and they were identified as Pseudomonas psychrotolerans,Chryseobacterium gleum and Bacillus subtilis by 16S rDNA amplification and sequencing. The plate inhibition zone method proved that there was no antagonism among the three kinds of biocontrol bacteria,and between the biocontrol bacteria and azadirachtin,thus they were suitable for compounding. Preliminary verification by pot experiment showed that the compound preparation treatment group from the filtrates by three kinds of biocontrol bacteria fermented for 48 h(the effective number of viable bacteria in the fermentation broth was more than 300 million/mL)and the azadirachtin diluent(1 000 times)was effective against root-knot nematodes The control effect of the disease reached 67.75%,and there was no significant difference in the control effect compared with the chemical pesticides(fluopyram and thiazophos)treatment group,and the tomato’s plant height,stem circumference,seedling and root biomass were all significantly increased. The preliminary conclusion from the potted experiment reveals that the compound scheme has the application potential to control tomato root-knot nematode disease.

Key words: Solanum lycopersicum, root-knot nematode, biological control, compound