生物技术通报 ›› 2021, Vol. 37 ›› Issue (8): 75-84.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0143

• 代谢生物学专题 • 上一篇    下一篇

以次生代谢物产量为目标的西兰花毛状根培养技术体系优化

张秀民1(), 马绍英2, 杨洁1, 包金玉1, 张晓玲1, 田鹏1, 路亚琦1, 李胜1()   

  1. 1.甘肃农业大学生命科学技术学院,兰州 730070
    2.甘肃农业大学基础实验教学中心,兰州 730070
  • 收稿日期:2021-02-03 出版日期:2021-08-26 发布日期:2021-09-10
  • 作者简介:张秀民,女,硕士研究生,研究方向:植物学;E-mail: 969885130@qq.com
  • 基金资助:
    国家自然科学基金项目(31860067);陇原青年创新与创业项目(2016-3-18);甘肃省民生科技项目(1603FCMG007);甘肃省教育厅项目(2017a-035)

Optimization of Hairy Roots Culture System of Broccoli Aiming at the Yield of Secondary Metabolites

ZHANG Xiu-min1(), MA Shao-ying2, YANG Jie1, BAO Jin-yu1, ZHANG Xiao-ling1, TIAN Peng1, LU Ya-qi1, LI Sheng1()   

  1. 1. College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070
    2. Basic Experimental Teaching Center of Gansu Agricultural University,Lanzhou 730070
  • Received:2021-02-03 Published:2021-08-26 Online:2021-09-10

摘要:

本研究旨在探究不同单因素条件下西兰花毛状根培养体系中萝卜硫苷(glucoraphanin,GRA)和萝卜硫素(sulforaphane,SF)释放的关系。通过10 mmol/L茉莉酸甲酯(methyl jasmonate,MeJA)处理,探究了在不同接种量、培养基体积、pH、培养温度及摇床转速下对西兰花毛状根中GRA和SF释放的影响;在单因素实验基础上,选取培养温度、毛状根接种量及pH进行响应面实验。结果表明:当培养温度为25.44℃,毛状根接种量为0.15 g,pH 5.56,西兰花毛状根中GRA和SF总产量为2.08 mg/flask,与回归模型预测理论总产量2.44 mg/flask相近;不同培养条件下,西兰花毛状根培养体系中GRA和SF释放至培养基中的量随之不同。

关键词: 西兰花毛状根, 萝卜硫苷, 萝卜硫素, 响应面法, 释放机理

Abstract:

This study focuses on the relationship between the release of glucoraphanin(GRA)and sulforaphane(SF)in broccoli hairy root culture system under different single factors. Through 10 mmol/L methyl jasmonate(MeJA)treatment,the effects of different inoculation amount,medium volume,pH,culture temperature and rotating speed on the release of GRA and SF in hairy roots were investigated. On the basis of single factor experiments,the culture temperature,hairy root inoculation amount and pH were subjected to response surface experiments. The results showed that total GRA and SF yields in the broccoli hairy roots were 2.08 mg under culture temperature of 25.44℃,hairy root inoculation amount of 0.15 g and pH of 5.56,which was close to the theoretical total yield of 2.44 mg predicted by regression model. The results demonstrated that the amount of GRA and SF released into the medium varied under different culture conditions.

Key words: hairy roots of broccoli, glucoraphanin, sulforaphane, response surface, release mechanism