农药残留检测关键用酶固定化研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2021-0080

摘要/Abstract

摘要：

为保障消费者食用安全,迫切需要研发农产品和食品中的农药残留快速检测技术。酶抑制法检测是目前农药残留快速检测技术中的主要研究方向之一,而酶的固定化是用基于酶抑制法原理对农药残留检测研究中的重要步骤。通过物理或化学的方法高效地将酶固定于载体上,同时保持酶的催化活性是开发各类基于酶抑制法检测农药残留传感器的关键。本文将从固定化酶方法、载体以及固定化酶产品在农药残留检测中的实际应用进行论述,为固定化酶在农药残留检测中的应用提供一定参考。

关键词: 酶抑制法, 农药残留, 固定化酶

Abstract:

In order to detect pesticide residues in agro-products and foods to ensure consumers’ food safety,some fast detection techniques need to be developed urgently. Enzyme inhibition method is one of current main research directions in the rapid detection techniques of pesticide residues. While the immobilized enzyme based on the principle of enzyme inhibition method is an important step in the research of detecting pesticide residue. Applying physical or chemical methods to efficiently fix enzyme on the carrier without losing catalytic activity is the key step to develop all kinds of sensor based on enzyme inhibition method to detect pesticide residues. In this paper,the immobilized enzyme methods,carriers and the practical applications of immobilized enzyme products in pesticide residue detection are reviewed,so as to provide some experiences and references for the applications of immobilized enzyme in pesticide residue detection.

Key words: enzyme inhibition method, pesticide residues, immobilized enzyme

朱永安, 王淼, 曹静, 喻鹤, 曹振, 金茂俊, 王静, 佘永新. 农药残留检测关键用酶固定化研究进展[J]. 生物技术通报, 2022, 38(1): 258-268.

ZHU Yong-an, WANG Miao, CAO Jing, YU He, CAO Zhen, JIN Mao-jun, WANG Jing, SHE Yong-xin. Research Progress in the Immobilization of Key Enzymes for Pesticides Residue Detection[J]. Biotechnology Bulletin, 2022, 38(1): 258-268.

图/表 5

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MOFs名称 MOFs name	固定化酶 Immobilized enzyme	检测农药 Detection of pesticide	线性范围 Linear range	检出限 LOD	参考文献 Reference
Pt@UiO66-NH₂	AChE	马拉硫磷Malathion	1×10^-14 -1×10^-9mol/L	4.9 × 10^-15 mol/L	[44]
MXene/AuNPs/MnO₂/Mn₃O₄/GCE	AChE	甲胺磷Methamidophos	1×10^-12 -1×10^-6mol/L	1.34 × 10^-13 mol/L	[45]
MOF-Basolite Z1200/μE	AChE	毒死蜱Chlorpyrifos	10-100 ng/L	6 ng/L	[46]
UiO-66-NH₂	OPH	甲基对硫磷Parathion-methyl	10-10⁶ng/mL	10 ng/mL	[47]
Ce-UiO-66/MWCNTs/GCE	AChE	对氧磷Paraoxon	0.01-150 nmol/L	0.004 nmol/L	[48]
Fe₃O₄NPs@ZIF-8	AChE	二嗪磷Dizinon	0.5-500 nmol/L	0.2 nmol/L	[49]
MOF-Pt	AChE	毒死蜱Chlorpyrifos	0.5 ng/mL-1.0 μg/mL	0.21 ng/mL	[50]
LDH@ZIF-8	AChE	二嗪磷Dizinon	0.5-300 nmol/L	0.22 nmol/L	[51]
Cu-Hemin MOFs/NECF	AChE	敌百虫Dipterex	0.25-20 ng/mL	0.082 ng/mL	[52]

MOFs名称 MOFs name	固定化酶 Immobilized enzyme	检测农药 Detection of pesticide	线性范围 Linear range	检出限 LOD	参考文献 Reference
Pt@UiO66-NH₂	AChE	马拉硫磷Malathion	1×10^-14 -1×10^-9mol/L	4.9 × 10^-15 mol/L	[44]
MXene/AuNPs/MnO₂/Mn₃O₄/GCE	AChE	甲胺磷Methamidophos	1×10^-12 -1×10^-6mol/L	1.34 × 10^-13 mol/L	[45]
MOF-Basolite Z1200/μE	AChE	毒死蜱Chlorpyrifos	10-100 ng/L	6 ng/L	[46]
UiO-66-NH₂	OPH	甲基对硫磷Parathion-methyl	10-10⁶ng/mL	10 ng/mL	[47]
Ce-UiO-66/MWCNTs/GCE	AChE	对氧磷Paraoxon	0.01-150 nmol/L	0.004 nmol/L	[48]
Fe₃O₄NPs@ZIF-8	AChE	二嗪磷Dizinon	0.5-500 nmol/L	0.2 nmol/L	[49]
MOF-Pt	AChE	毒死蜱Chlorpyrifos	0.5 ng/mL-1.0 μg/mL	0.21 ng/mL	[50]
LDH@ZIF-8	AChE	二嗪磷Dizinon	0.5-300 nmol/L	0.22 nmol/L	[51]
Cu-Hemin MOFs/NECF	AChE	敌百虫Dipterex	0.25-20 ng/mL	0.082 ng/mL	[52]

传感器名称 Sensor name	类型 Type	农药 Pesticide	线性范围 Linear range	检出限 LOD	参考文献 Reference
AChE/e-pGON/GCE	电化学生物传感器 Electrochemical biosensor	甲萘威 Carbaryl	0.3-6.1 ng/mL	0.15 ng/mL	[59]
AChE/Au/ssDNA- SWCNT/PANI	电化学生物传感器 Electrochemical biosensor	甲基对硫磷 Parathion-methyl 毒死蜱 Chlorpyrifos	1.0×10^-11-1.0×10^-6mol/L	1.0×10^-12mol/L	[60]
Tyr-BCs/Au	电化学发光传感器 ECL	阿特拉津 Atrazine	0.0001-0.01 μg/L 0.01-20 μg/L	0.08 ng/L	[31]
ACh/CNT/NH₂	生物传感器 Biosensor	对氧磷 Paraoxon	0.2-1 nmol/L	0.08 nmol/L	[34]
AChE/GMA/EDMA	微流控芯片 Micro-fluidic chip	对氧磷 Paraoxon	0.25-2.5 mg/mL	0.17 mg/mL	[61]
AChE-FH-A-GNPs	生物传感器 Biosensor	克百威 Carbofuran 杀线威 Oxamyl 灭多威 Methomyl 甲萘威 Carbaryl	NR	2 nmol/L 21 nmol/L 113 nmol/L 236 nmol/L	[62]
AChE/Ce-UiO-66 /MWCNTs/GCE	电化学生物传感器 Electrochemical biosensor	对氧磷 Paraoxon	0.01-150 nmol/L	0.004 nmol/L	[48]
AChE/AuPt/PDA	生物传感器 Biosensor	对氧磷 Paraoxon	0.5-1000 ng/L	0.185 ng/L	[63]
GCE/P-ABSA/DAR /AuNPs/DAR/AChE	生物传感器 Biosensor	马拉硫磷 Malathion 甲基对硫磷 Parathion-methyl	0.003-30 pmol/L 0.0038-38 pmol/L	0.0016 pmol/L 0.0022 pmol/L	[9]
AChE/PET/Ag/AgCl	微流控芯片 Micro-fluidic chip	对硫磷 Parathion	1.0×10^-7-1.0×10^-5 g/mL	3.3×10^-8 g/mL	[12]
NF/AChE-CS/SNS-NF/GCE	生物传感器 Biosensor	甲基对硫磷 Parathion-methyl 毒死蜱 Chlorpyrifos 克百威 Carbofuran	10^-12-10^-10 mol/L 10^-10-10^-8mol/L	5.0×10^-13mol/L	[16]

传感器名称 Sensor name	类型 Type	农药 Pesticide	线性范围 Linear range	检出限 LOD	参考文献 Reference
AChE/e-pGON/GCE	电化学生物传感器 Electrochemical biosensor	甲萘威 Carbaryl	0.3-6.1 ng/mL	0.15 ng/mL	[59]
AChE/Au/ssDNA- SWCNT/PANI	电化学生物传感器 Electrochemical biosensor	甲基对硫磷 Parathion-methyl 毒死蜱 Chlorpyrifos	1.0×10^-11-1.0×10^-6mol/L	1.0×10^-12mol/L	[60]
Tyr-BCs/Au	电化学发光传感器 ECL	阿特拉津 Atrazine	0.0001-0.01 μg/L 0.01-20 μg/L	0.08 ng/L	[31]
ACh/CNT/NH₂	生物传感器 Biosensor	对氧磷 Paraoxon	0.2-1 nmol/L	0.08 nmol/L	[34]
AChE/GMA/EDMA	微流控芯片 Micro-fluidic chip	对氧磷 Paraoxon	0.25-2.5 mg/mL	0.17 mg/mL	[61]
AChE-FH-A-GNPs	生物传感器 Biosensor	克百威 Carbofuran 杀线威 Oxamyl 灭多威 Methomyl 甲萘威 Carbaryl	NR	2 nmol/L 21 nmol/L 113 nmol/L 236 nmol/L	[62]
AChE/Ce-UiO-66 /MWCNTs/GCE	电化学生物传感器 Electrochemical biosensor	对氧磷 Paraoxon	0.01-150 nmol/L	0.004 nmol/L	[48]
AChE/AuPt/PDA	生物传感器 Biosensor	对氧磷 Paraoxon	0.5-1000 ng/L	0.185 ng/L	[63]
GCE/P-ABSA/DAR /AuNPs/DAR/AChE	生物传感器 Biosensor	马拉硫磷 Malathion 甲基对硫磷 Parathion-methyl	0.003-30 pmol/L 0.0038-38 pmol/L	0.0016 pmol/L 0.0022 pmol/L	[9]
AChE/PET/Ag/AgCl	微流控芯片 Micro-fluidic chip	对硫磷 Parathion	1.0×10^-7-1.0×10^-5 g/mL	3.3×10^-8 g/mL	[12]
NF/AChE-CS/SNS-NF/GCE	生物传感器 Biosensor	甲基对硫磷 Parathion-methyl 毒死蜱 Chlorpyrifos 克百威 Carbofuran	10^-12-10^-10 mol/L 10^-10-10^-8mol/L	5.0×10^-13mol/L	[16]