生物技术通报 ›› 2022, Vol. 38 ›› Issue (5): 248-256.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0862

• 技术与方法 • 上一篇    下一篇

噬菌体抗体展示技术及其在抗新冠病毒抗体发现中的应用

王加利(), 和似琦, 康子茜, 王建勋()   

  1. 北京中医药大学生命科学学院,北京 102400
  • 收稿日期:2021-07-04 出版日期:2022-05-26 发布日期:2022-06-10
  • 作者简介:王加利,女,博士,研究方向:噬菌体展示;E-mail: wangjl120@163.com
  • 基金资助:
    教育部双一流引导专项师资队伍建设项目(1000061020014)

Antibody Phage Display Technology and Its Application in the Discovery of Anti-SARS-CoV-2 Antibodies

WANG Jia-li(), HE Si-qi, KANG Zi-xi, WANG Jian-xun()   

  1. School of Life Sciences,Beijing University of Chinese Medicine,Beijing 102400
  • Received:2021-07-04 Published:2022-05-26 Online:2022-06-10

摘要:

噬菌体抗体展示技术是第一个也是目前应用最广泛的体外抗体筛选技术,可用于发现治疗各种疾病的全人源抗体。虽然存在不同的抗体发现方法,但噬菌体抗体展示技术已成为发现和优化靶标特异性单克隆抗体不可或缺的工具。研究人员可通过在丝状噬菌体上创建组合抗体库,从而在几周内获得抗原特异性抗体。在当前冠状病毒病(COVID-19)大流行的情况下,对中和抗体的研究激励着研究人员寻找出抗SARS-CoV-2的候选治疗药物。通过对噬菌体抗体库的筛选,目前已有许多不同的SARS-CoV-2中和抗体被发现。因此,本文综述了噬菌体抗体展示技术的原理,抗体库的构建、分类及淘选流程,并对其优点及局限性进行了讨论。此外,还总结了利用该技术发现抗SARS-CoV-2中和抗体的最新进展。旨为今后该技术在抗体发现中的应用提供理论支持。

关键词: 单克隆抗体, 噬菌体抗体展示, 抗体库, 淘选, 新冠病毒

Abstract:

Phage antibody display technology is the first and most widely used of the in vitro antibody screening technologies,which enable the discovery of human antibodies for therapeutic applications associated with all kinds of diseases. Different antibody discovery approaches exist,but phage antibody display technology has become an indispensable tool for the discovery and optimization of target-specific monoclonal antibodies. The researchers can create combinatorial antibody libraries on filamentous phage to be utilized for generating antigen specific antibodies in a matter of weeks. In the current situation of pandemic of Coronavirus disease(COVID-19),the research for neutralizing antibodies is motivating the researchers to find therapeutic candidates against SARS-CoV-2. By panning phage antibody libraries,many different SARS-CoV-2 neutralizing antibodies have been found at present. Therefore,we summarize the principles of phage antibody display technology,and the construction,classification and panning process of phage antibody library,also discuss the advantages and limitations. Furthermore,we summarize the recent advances in the use of this technology for discovering neutralizing antibody against SARS-CoV-2. It is aimed to provide the theoretical support for the application of this technology in antibody discovery in the coming years.

Key words: monoclonal antibody, phage antibody display, antibody library, panning, SARS-CoV-2