生物技术通报 ›› 2022, Vol. 38 ›› Issue (7): 224-235.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1251

• 研究报告 • 上一篇    下一篇

盐胁迫条件下哈茨木霉ST02对椒样薄荷生长及根区土壤理化性质的影响

赵忠娟1(), 杨凯1, 扈进冬1, 魏艳丽1, 李玲1, 徐维生2, 李纪顺1()   

  1. 1.山东省应用微生物重点实验室 山东省科学院生态研究所 齐鲁工业大学(山东省科学院),济南 250103
    2.山东省沂水县诸葛镇农业服务中心,临沂 276422
  • 收稿日期:2021-09-28 出版日期:2022-07-26 发布日期:2022-08-09
  • 作者简介:赵忠娟,女,博士,研究方向:盐渍土壤的微生物-植物联合修复;E-mail: zzjfrances@aliyun.com
  • 基金资助:
    山东省自然科学基金(ZR2019BC076);山东省自然科学基金(ZR2020QC044);山东省重大科技创新工程(2019JZZY010723);山东省科学院院地产学研协同创新基金(2019-CXY5)

Effects of Trichoderma harzianum ST02 on the Growth of Peppermint and Physicochemical Properties of Root Zone Soil Under Salt Stress

ZHAO Zhong-juan1(), YANG Kai1, HU Jin-dong1, WEI Yan-li1, LI Ling1, XU Wei-sheng2, LI Ji-shun1()   

  1. 1. Shandong Province Key Laboratory of Applied Microbiology,Ecology Institute of Shandong Academy of Sciences,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250103
    2. Zhuge Agricultural Service Center,Yishui County,Shandong Province,Linyi 276422
  • Received:2021-09-28 Published:2022-07-26 Online:2022-08-09

摘要:

为研究耐盐木霉对植物耐盐性及盐渍土壤改良的作用,以耐盐哈茨木霉ST02和耐盐椒样薄荷为研究对象,分析盐胁迫条件下ST02对椒样薄荷生理生长、椒样薄荷根区土壤理化性质和土壤中酶活性大小的影响;并通过椒样薄荷生理生长与土壤因子相关性分析和主成分分析,分析影响椒样薄荷生理生长的主要土壤因子。结果表明,ST02缓解盐胁迫对椒样薄荷生长和光合作用的抑制,促进盐胁迫条件下椒样薄荷的生长和光合作用强度;NaCl胁迫条件下ST02减轻椒样薄荷细胞中丙二醛(malondialdehyde,MDA)、可溶性蛋白(soluble protein,SP)和脯氨酸(proline,Pro)的积累,增强超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)和过氧化氢酶(catalase,CAT)活性,通过抗氧化防御途径增强椒样薄荷耐盐性;NaCl胁迫条件下,椒样薄荷生理生长综合指数(physiological/growth comprehensive index,PGCI)为-2.391,ST02处理促进椒样薄荷生理生长(指数为-0.025);NaCl胁迫条件下,ST02能影响土壤理化性质,降低根区土壤盐浓度(降低17.36%),并显著提高土壤中碱解氮(alkali-hydrolyzed nitrogen,AN)的含量;ST02能够影响NaCl胁迫条件下椒样薄荷根区土壤酶活性,显著提高土壤中纤维素酶(soil cellulase,SCL)和过氧化氢酶(soil catalase,SCAT)活性;通过椒样薄荷根区土壤理化性质与椒样薄荷生理生长的相关性分析,表明土壤理化性质和土壤酶活性影响椒样薄荷的生长与生理性状;主成分分析影响椒样薄荷生理生长最主要的土壤因子为:EC(electrical conductance)>SCL>SSC(soil saccharase)/SNEP(soil neutrality phosphatase)>AP(available phosphorus)>pH。

关键词: 耐盐木霉, 耐盐植物, 椒样薄荷, 生理生长指数, 土壤理化性质, 土壤酶活性

Abstract:

The salt-resistant Trichoderma harzianum strain ST02 and salt-resistant peppermint were used in this study to analyze the effects of Trichoderma on plant salt resistance and saline soil improvement. The physiological and growth traits of peppermint,the soil physicochemical property and soil enzyme activity of root zone soil under salt stress were analyzed. And the main soil factors related to the physiological and growth of peppermint were analyzed through the correlation analysis and principal component analysis(PCA). The results showed ST02 relieved the inhibition of NaCl on peppermint growth and photosynthesis and promoted its growth and photosynthesis intensity under salt stress. When treated with NaCl,ST02 reduced the accumulation of maldehyde aldehyde(MDA),soluble protein(SP)and proline(Pro)in peppermint cells,enhanced superoxide dismutase(SOD),peroxide dismutase(POD),and catalase(CAT)activities,and enhanced the salt resistance of peppermint through antioxidant response. The physiological/growth comprehensive index(PGCI)of peppermint treated with NaCl was -2.391,ST02 promoted the physiological and growth of peppermint,as index was -0.025. ST02 influenced the soil physicochemical properties for root zone soil of peppermint under NaCl stress,reduced salinity by 17.36%,and significantly enhanced the content of alkali-hydrolyzed nitrogen(AN). The soil enzyme activity for the root zone soil of peppermint was also affected by ST02 strain,and the activity of soil cellulase(SCL)and catalase(SCAT)increased remarkably. The soil physicochemical property and soil enzyme activity affected the physiological and growth traits under NaCl stress through the correlation analysis. And the main soil factor for physiological and growth of peppermint was:EC(electrical conductance)> SCL >SSC(soil saccharase)/SNEP(soil neutrality phosphatase)>AP(available phosphorus)>pH.

Key words: salt-resistant Trichoderma, salt-resistant plant, Mentha×piperita L., physiological and growth index, soil physicochemical property, soil enzyme activity