生物技术通报 ›› 2022, Vol. 38 ›› Issue (12): 137-143.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0238

• 综述与专论 • 上一篇    下一篇

Vero细胞基质流感疫苗研究进展

马芳芳1,3(), 康碧静1,3, 马春英1,3, 刘振斌1,2, 杨迪1,2, 乔自林1,2, 王明明1,3, 马忠仁1,2, 王家敏1,2()   

  1. 1.西北民族大学生物医学研究中心 甘肃省动物细胞技术创新中心,兰州 730030
    2.西北民族大学生物工程与技术国家民委重点实验室,兰州 730030
    3.西北民族大学生命科学与工程学院,兰州 730030
  • 收稿日期:2022-02-26 出版日期:2022-12-26 发布日期:2022-12-29
  • 作者简介:马芳芳,硕士研究生,研究方向:动物细胞培养工程;E-mail:3535205030@qq.com
  • 基金资助:
    教育部动物医学生物工程创新团队(IRT_17R88);西北民族大学中央高校基本科研业务费专项资金项目(31920210053)

Research Progress in Vero Cell-based Influenza Vaccine

MA Fang-fang1,3(), KANG Bi-jing1,3, MA Chun-ying1,3, LIU Zhen-bin1,2, YANG Di1,2, QIAO Zi-lin1,2, WANG Ming-ming1,3, MA Zhong-ren1,2, WANG Jia-min1,2()   

  1. 1. Gansu Tech Innovation Center of Animal Cell,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030
    2. Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030
    3. Life Science and Engineering College of Northwest Minzu University,Lanzhou 730030
  • Received:2022-02-26 Published:2022-12-26 Online:2022-12-29

摘要:

流感防控是人类至今面临的严峻挑战之一,流感疫苗接种是预防流感的关键。由于传统的流感疫苗生产工艺依赖鸡胚生产而存在局限性,规模化流感疫苗生产基质逐渐从鸡胚转为哺乳动物细胞,目前用于流感疫苗研发的常见细胞系有MDCK、Vero和PER.C6。其中Vero细胞低代次无成瘤性且已用于多种人用病毒性疫苗生产,是WHO推荐的广泛用于人和动物病毒性疫苗生产的首选细胞系,但Vero细胞基质流感疫苗在生产中仍面临挑战。现就流感疫苗研究、细胞基质流感疫苗的综合比较及Vero细胞在流感疫苗生产中所面临的困难予以综述,旨在为细胞基质流感疫苗研发提供参考。

关键词: 流感, Vero细胞, 疫苗, 悬浮培养

Abstract:

The prevention of influenza is one of the most severe challenges faced by mankind. Influenza vaccine inoculation is the key of preventing influenza. There are limitations in the traditional production process of influenza vaccine due to its dependence on chicken embryo, and the production of large-scale influenza vaccine is gradually transferred from chicken embryo to mammalian cells. Currently, MDCK, Vero and PER.C6 cell lines can be used for influenza vaccine development. Vero cells have non-tumogenesis within a certain range of generations and been used in the production of a variety of human viral vaccines. It is the cell line recommended by WHO that is widely used in the production of human and animal virial vaccines, however, there is still challenges in the Vero cell-based influenza vaccine production. This paper reviews the research of influenza vaccine, the comprehensive comparison of cell-based influenza vaccine and the difficulties faced by Vero cells in the production of influenza vaccine, aiming to provide reference for the development of cell-based influenza vaccine.

Key words: influenza, Vero cell, vaccine, suspension culture