生物技术通报 ›› 2023, Vol. 39 ›› Issue (10): 246-255.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0590

• 研究报告 • 上一篇    下一篇

乌头内生细菌JY-3-1R的鉴定及其生防和促生能力研究

邹兰1(), 王茜1, 李慕仪1, 叶坤浩2, 黄晶1()   

  1. 1.西南科技大学生命科学与工程学院,绵阳 621010
    2.绵阳市农业科学研究院中药材研究所,绵阳 621023
  • 收稿日期:2023-06-21 出版日期:2023-10-26 发布日期:2023-11-28
  • 通讯作者: 黄晶,女,博士,教授,研究方向:中药材生态栽培;E-mail: huang.jing@swust.edu.cn
  • 作者简介:邹兰,女,博士,讲师,研究方向:植物病害生物防治;E-mail: zoulan@swust.edu.cn
  • 基金资助:
    四川省自然科学基金项目(2022NSFSC1728);四川省科技计划项目(2023YFS0346)

Identification, Biocontrol and Plant Growth-promoting Potential of Endophytic Bacterial Strain JY-3-1R from Aconitum carmichaelii Debx.

ZOU Lan1(), WANG Qian1, LI Mu-yi1, YE Kun-hao2, HUANG Jing1()   

  1. 1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010
    2. Institute of Traditional Medicinal Materials, Mianyang Academy of Agricultural Science, Mianyang 621023
  • Received:2023-06-21 Published:2023-10-26 Online:2023-11-28

摘要:

分离筛选具生防和促生潜力的乌头内生细菌,为乌头土传病害防治提供优质微生物菌种。采用纯培养法从健康乌头分离内生细菌,对峙试验检测菌株拮抗病原菌能力,多位点持家基因序列鉴定菌株分类地位,平板法检测菌株产酶和铁载体能力,比色法检测菌株产IAA能力,大田试验检测菌株对乌头生长的影响和白绢病的生防潜力。从111株乌头内生细菌中筛选到1株对白绢病和根腐病病原菌有显著抑制作用的内生细菌JY-3-1R,该菌鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。JY-3-1R无细胞发酵滤液对齐整小核菌(Sclerotium rolfsii)菌丝生长和菌核萌发抑制率达100%。该菌具产IAA、铁载体、蛋白酶、纤维素酶和葡聚糖酶的能力,同时包含合成伊枯草菌素、丰原素、表面活性素、芽孢菌霉素、杆菌溶素、杆菌烯和大环内酯的功能基因。大田条件下,JY-3-1R接种降低乌头白绢病发病率高达40%,生防效率为61.53%-84.61%,生防能力持续30 d,同时植株茎、主根和子根干重分别提高了34.34%、82.59%和56.08%。JY-3-1R兼具生防和促生能力,具有开发作为乌头生物肥料和生防材料的潜力。

关键词: 内生细菌, 生防, 促生, 白绢病

Abstract:

In order to provide promising microbial resource for the control of soil-borne diseases in Aconitum carmichaelii Debx., endophytic bacterial strains with biocontrol and plant growth promoting potential were isolated and screened from healthy A. carmichaelii plants. Pure culture method was used to isolate endophytic bacteria, and dual-culture method was to check the antagonistic activity of candidate strains against pathogens. Multi-locus housekeeping gene sequence analysis was to investigate phylogeny of the strain. Enzyme- and siderophore-producing capacities of bacterial strain were measured on plates. Salkowski method was applied to measure indoleacetic acid(IAA)-producing ability of the strain. Field experiment was employed to demonstrate plant growth promoting ability and biocontrol potential against southern blight in A. carmichaelii. One strain JY-3-1R screened from 111 A. carmichaelii endophytic bacteria showed significant antagonistic activity against Sclerotium rolfsii and Fusarium oxysporum in vitro. The JY-3-1R was identified as Bacillus amyloliquefaciens. Cell-free culture filtrate of JY-3-1R presented 100% inhibition on the hyphal growth and sclerotia germination of S. rolfsii. JY-3-1R was able to produce IAA, siderophore, protease, cellulase, glucanase, also had the functional genes of synthesizing iturin, fengyin, surfactin, bacillomycin, bacilysin, bacillaene, and macrolactin. Under field conditions, JY-3-1R inoculation reduced around 40% of southern blight occurrence, whose biocontrol rates ranged from 61.53% to 84.61%. This biocontrol efficiency lasted at least for 30 d. Meanwhile, JY-3-1R inoculation promoted 34.34%, 82.59% and 56.08% of stem, main root and lateral root dry weight, respectively of A. carmichaelii. Altogether, JY-3-1R demonstrated both plant growth promoting and biocontrol potential, and could be used as good candidate of biofertilizer and biocontrol agent of A. carmichaelii.

Key words: endophytic bacteria, biocontrol, plant growth promotion, southern blight