石珊瑚共附生真菌次级代谢产物的抗炎活性及化学多样性研究

doi:10.13560/j.cnki.biotech.bull.1985.2023-0501

生物技术通报 ›› 2023, Vol. 39 ›› Issue (12): 261-275.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0501

石珊瑚共附生真菌次级代谢产物的抗炎活性及化学多样性研究

廖清楠¹(), 周龙建¹^,²^,³, 杨志友¹^,²^,³, 冯昀铠¹, 黄谊君¹, 胡雪琼¹^,³, 张翼¹^,²^,³(), 刘亚月¹^,²^,³()

1.广东海洋大学食品科技学院广东省水产品加工与安全重点实验室广东海洋大学深圳研究院海洋医药研发中心湛江市脑健康海洋药物与营养品重点实验室，湛江 524088
2.南方海洋科学与工程广东省实验室（湛江），湛江 524006
3.海洋食品精深加工关键技术省部共建协同创新中心大连工业大学，大连 116034

收稿日期:2023-05-26 出版日期:2023-12-26 发布日期:2024-01-11
通讯作者: 刘亚月，女，博士，讲师，研究方向：南海海洋活性物质; E-mail: yayue_liu@163.com；
张翼，男，博士，教授，研究方向：海洋天然产物；E-mail: hubeizhangyi@163.com
作者简介:廖清楠，女，硕士研究生，研究方向：南海海洋活性物质; E-mail: 17854225126@163.com
基金资助:
湛江市科技发展专项资金竞争性分配项目(2020A01030);广东省基础与应用基础研究基金自然科学基金(2022A1515010783);广东省科技专项资金-基础与应用基础研究专题(2021A05240);广东省普通高校重点领域专项（生物医药与健康）(2021ZDZX2064);广东海洋大学博士科研启动项目(R18008);广东海洋大学博士科研启动项目(060302042201)

Studies on Anti-inflammatory Activity and Chemical Diversity of Secondary Metabolites from Symbiotic Fungi in Stony Corals

LIAO Qing-nan¹(), ZHOU Long-jian¹^,²^,³, YANG Zhi-you¹^,²^,³, FENG Yun-kai¹, HUANG Yi-jun¹, HU Xue-qiong¹^,³, ZHANG Yi¹^,²^,³(), LIU Ya-yue¹^,²^,³()

1. College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Center for Marine Drugs Research & Development, Shenzhen Institute of Guangdong Ocean University, Zhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain Health, Zhanjiang 524088
2. Southern Marine Science and Engineering Guangdong Laboratory（Zhanjiang）, Zhangjiang 524006
3. Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034

Received:2023-05-26 Published:2023-12-26 Online:2024-01-11

摘要/Abstract

摘要：

以石珊瑚共附生真菌为研究对象，探究不同培养条件对其次级代谢产物抗炎活性和化学多样性的影响。基于OSMAC（one stain many compounds）策略，分别选用马铃薯葡萄糖水（potato dextrose broth, PDB）和糙米培养基，并设置 3 种盐度（0.3%、3.0% 和 10.0%），对 31 株石珊瑚来源共附生真菌进行小规模发酵培养。以脂多糖（lipopolysaccharide, LPS）诱导的BV-2小胶质细胞为炎症模型，从中筛选出具有抗炎活性的菌株；并通过薄层色谱法（thin-layer chromatography, TLC）指纹图谱、超高效液相色谱-四极杆飞行时间质谱法（ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry, UPLC-QTOF-MS）以及基于特征的分子网络（feature-based molecular networking, FBMN）等技术手段进一步分析抗炎活性菌株次级代谢产物的化学多样性。结果表明，5株菌株在糙米培养基条件下表现出显著的抗炎活性，对LPS诱导的BV-2细胞中一氧化氮（nitric oxide, NO）的释放显示中等或强的抑制活性，且呈现显著的浓度剂量依赖性。而菌株C3-9粗提物在10和20 µg/mL的低浓度条件下也表现出了显著的抑制NO产生的作用，进一步的TLC和FBMN分析显示菌株C3-9的次级代谢产物中含有丰富的抗炎活性化合物，且具有高度的多样性。石珊瑚共附生真菌C3-9次级代谢产物具有显著的抗炎活性和多样性，为后续抗炎活性化合物的发现提供了基础。

关键词: 石珊瑚, 共附生真菌, OSMAC, 抗炎活性, 化学多样性

Abstract:

Stony coral symbiotic fungi were used to investigate the effects of different culture conditions on the anti-inflammatory activities and chemical diversities of their secondary metabolites. Based on the OSMAC（one stain many compounds）strategy, 31 strains of stony coral-derived symbiotic fungi were cultured in small-scale fermentation with potato dextrose broth（PDB）and brown rice medium at three salinities（0.3%, 3.0%, and 10.0%）, respectively. Lipopolysaccharide（LPS）-induced BV-2 microglia were used as the inflammation model, from which the strains with anti-inflammatory activity were selected. Via thin-layer chromatography（TLC）,ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry（UPLC-QTOF-MS）and feature-based molecular networking（FBMN）, the chemical diversity of the secondary metabolites from the anti-inflammatory active strains was further analyzed. The results showed that five strains demonstrated significant anti-inflammatory activity under the brown rice medium conditions and showed moderate or strong inhibitory activity against LPS-induced nitric oxide（NO）release in BV-2 cells, with a significant concentration-dose dependence. While the crude extract of strain C3-9 also showed the significant inhibition to NO production at low concentrations of 10 and 20 µg/mL, further TLC and FBMN analyses revealed that the secondary metabolites of strain C3-9 were rich in anti-inflammatory compounds and highly diverse. The secondary metabolites of the stony coral co-epiphytic fungus C3-9 have significant anti-inflammatory activity and diversity, providing a basis for the subsequent discovery of anti-inflammatory active compounds.

Key words: stony coral, symbiotic fungi, OSMAC, anti-inflammatory activity, chemical diversity

廖清楠, 周龙建, 杨志友, 冯昀铠, 黄谊君, 胡雪琼, 张翼, 刘亚月. 石珊瑚共附生真菌次级代谢产物的抗炎活性及化学多样性研究[J]. 生物技术通报, 2023, 39(12): 261-275.

LIAO Qing-nan, ZHOU Long-jian, YANG Zhi-you, FENG Yun-kai, HUANG Yi-jun, HU Xue-qiong, ZHANG Yi, LIU Ya-yue. Studies on Anti-inflammatory Activity and Chemical Diversity of Secondary Metabolites from Symbiotic Fungi in Stony Corals[J]. Biotechnology Bulletin, 2023, 39(12): 261-275.

图/表 10

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菌株编号Strain No.	菌属Bacterial genus	珊瑚来源Coral source
C1-5	旋孢腔菌属Cochliobolus sp.	普哥滨珊瑚Porites pukoensis
C3-1cc	-	牡丹珊瑚Pavona Lamarck
C3-3	土曲霉Aspergillus terreus	牡丹珊瑚P. Lamarck
C3-4	土曲霉A. terreus	牡丹珊瑚P. Lamarck
C3-5	肉座菌属Hypocreaceae sp.	牡丹珊瑚P. Lamarck
C3-7	土曲霉A. terreus	牡丹珊瑚P. Lamarck
C3-8	青霉属Penicillium citrinum	牡丹珊瑚P. Lamarck
C3-9	土曲霉A. terreus	牡丹珊瑚P. Lamarck
C14-2B	-	丛生盔形珊瑚Galaxea fascicularis
C14-2	土曲霉A. terreus	丛生盔形珊瑚G. fascicularis
C14-5	镰刀霉属Fusarium sp.	丛生盔形珊瑚G. fascicularis
C14-7	座囊霉菌属Dothideales sp.	丛生盔形珊瑚G. fascicularis
C14-8	Curreya austroafricana	丛生盔形珊瑚G. fascicularis
C14-9	土曲霉A. terreus	丛生盔形珊瑚G. fascicularis
C14-10	透孢黑团壳Massarina sp.	丛生盔形珊瑚G. fascicularis
C14-12	赤散囊菌Eurotium rubrum	丛生盔形珊瑚G. fascicularis
C14-13	节孢霉属Arthrinium sp.	丛生盔形珊瑚G. fascicularis
C14-17	葡萄穗霉属Stachybotrys sp.	丛生盔形珊瑚G. fascicularis
C14-18	土曲霉A. terreus	丛生盔形珊瑚G. fascicularis
C14-19	葡萄穗霉属Stachybotrys sp.	丛生盔形珊瑚G. fascicularis
C21-1	青霉属P. citrinum	普哥滨珊瑚Porites pukoensis
C21-2	土曲霉A. terreus	普哥滨珊瑚P. pukoensis
C21-5	旋孢腔菌属Cochliobolus sp.	普哥滨珊瑚P. pukoensis
C21-6	青霉属P. citrinum	普哥滨珊瑚P. pukoensis
C21-8	枝顶孢属Acremonium persicinum	普哥滨珊瑚P. pukoensis
C21-11	土曲霉A. terreus	普哥滨珊瑚P. pukoensis
C21-13	赤霉属Gibberella sp.	普哥滨珊瑚P. pukoensis
C21-17	蕉孢壳属Eutypella sp.	普哥滨珊瑚P. pukoensis
C21-18	-	普哥滨珊瑚P. pukoensis
C21-19	弯孢属Curvularia sp.	普哥滨珊瑚P. pukoensis
C21-20	旋孢腔菌属Cochliobolus sp.	普哥滨珊瑚P. pukoensis

菌株编号Strain No.	菌属Bacterial genus	珊瑚来源Coral source
C1-5	旋孢腔菌属Cochliobolus sp.	普哥滨珊瑚Porites pukoensis
C3-1cc	-	牡丹珊瑚Pavona Lamarck
C3-3	土曲霉Aspergillus terreus	牡丹珊瑚P. Lamarck
C3-4	土曲霉A. terreus	牡丹珊瑚P. Lamarck
C3-5	肉座菌属Hypocreaceae sp.	牡丹珊瑚P. Lamarck
C3-7	土曲霉A. terreus	牡丹珊瑚P. Lamarck
C3-8	青霉属Penicillium citrinum	牡丹珊瑚P. Lamarck
C3-9	土曲霉A. terreus	牡丹珊瑚P. Lamarck
C14-2B	-	丛生盔形珊瑚Galaxea fascicularis
C14-2	土曲霉A. terreus	丛生盔形珊瑚G. fascicularis
C14-5	镰刀霉属Fusarium sp.	丛生盔形珊瑚G. fascicularis
C14-7	座囊霉菌属Dothideales sp.	丛生盔形珊瑚G. fascicularis
C14-8	Curreya austroafricana	丛生盔形珊瑚G. fascicularis
C14-9	土曲霉A. terreus	丛生盔形珊瑚G. fascicularis
C14-10	透孢黑团壳Massarina sp.	丛生盔形珊瑚G. fascicularis
C14-12	赤散囊菌Eurotium rubrum	丛生盔形珊瑚G. fascicularis
C14-13	节孢霉属Arthrinium sp.	丛生盔形珊瑚G. fascicularis
C14-17	葡萄穗霉属Stachybotrys sp.	丛生盔形珊瑚G. fascicularis
C14-18	土曲霉A. terreus	丛生盔形珊瑚G. fascicularis
C14-19	葡萄穗霉属Stachybotrys sp.	丛生盔形珊瑚G. fascicularis
C21-1	青霉属P. citrinum	普哥滨珊瑚Porites pukoensis
C21-2	土曲霉A. terreus	普哥滨珊瑚P. pukoensis
C21-5	旋孢腔菌属Cochliobolus sp.	普哥滨珊瑚P. pukoensis
C21-6	青霉属P. citrinum	普哥滨珊瑚P. pukoensis
C21-8	枝顶孢属Acremonium persicinum	普哥滨珊瑚P. pukoensis
C21-11	土曲霉A. terreus	普哥滨珊瑚P. pukoensis
C21-13	赤霉属Gibberella sp.	普哥滨珊瑚P. pukoensis
C21-17	蕉孢壳属Eutypella sp.	普哥滨珊瑚P. pukoensis
C21-18	-	普哥滨珊瑚P. pukoensis
C21-19	弯孢属Curvularia sp.	普哥滨珊瑚P. pukoensis
C21-20	旋孢腔菌属Cochliobolus sp.	普哥滨珊瑚P. pukoensis

粗提物编号/浓度 Crude extract No./ Concentration/（µg·mL^-1）	盐度为0.3% Salinity of 0.3%					盐度为3% Salinity of 3%					盐度为10% Salinity of 10%
粗提物编号/浓度 Crude extract No./ Concentration/（µg·mL^-1）	1	10	20	50	100	1	10	20	50	100	1	10	20	50	100
C1-5	-	-	-	-	-	-	-	-	***	****	-	-	****	****	****
C3-1cc	-	-	-	-	-	-	-	-	-	-	-	-	-	****	-
C3-3	-	-	-	-	-	-	-	-	-	-	-	-	**	-	-
C3-4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C3-5	-	-	-	-	-	-	-	-	-	-	-	-	-	-	*
C3-7	-	-	-	-	-	-	-	-	-	-	-	-	-	****	-
C3-8	-	-	-	-	-	-	-	-	*	-	-	-	-	-	***
C3-9	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C14-2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C14-2B	-	-	-	-	-	-	-	-	-	-	-	-	-	***	-
C14-5	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C14-7	-	-	-	-	-	-	-	-	****	****	**	-	-	*	-
C14-8	-	-	-	-	-	-	-	-	-	-	-	-	-	-	*
C14-9	-	-	-	-	-	-	-	-	****	****	-	-	-	-	**
C14-10	-	-	-	-	-	-	-	-	****	****	-	-	-	****	****
C14-12	-	-	-	-	-	-	*	**	***	****	/	/	/	/	/
C14-13	-	-	**	****	****	-	-	-	-	-	-	-	-	-	-
C14-16	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C14-17	-	-	-	-	-	-	-	-	-	-	-	-	-	***	****
C14-18	-	-	****	****	****	-	-	-	-	-	-	-	-	-	-
C14-19	-	-	-	-	-	-	*	-	-	-	-	-	****	****	-
C21-1	-	-	-	-	-	-	-	-	*	****	-	***	****	-	-
C21-2	-	-	-	-	-	-	-	-	-	-	-	****	-	-	-
C21-6	-	-	-	-	-	-	-	-	-	-	/	/	/	/	/
C21-8	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C21-11	-	-	-	-	-	-	-	-	-	-	/	/	/	/	/
C21-13	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C21-17	-	-	-	-	-	-	-	-	-	-	/	/	/	/	/
C21-18	-	-	-	-	-	-	*	***	-	-	-	-	**	***	****
C21-19	-	-	-	-	-	-	-	-	-	-	-	-	-	-	****
C21-20	-	-	-	-	-	-	-	-	-	-	-	-	-	****	****

粗提物编号/浓度 Crude extract No./ Concentration/（µg·mL^-1）	盐度为0.3% Salinity of 0.3%					盐度为3% Salinity of 3%					盐度为10% Salinity of 10%
粗提物编号/浓度 Crude extract No./ Concentration/（µg·mL^-1）	1	10	20	50	100	1	10	20	50	100	1	10	20	50	100
C1-5	-	-	-	-	-	-	-	-	***	****	-	-	****	****	****
C3-1cc	-	-	-	-	-	-	-	-	-	-	-	-	-	****	-
C3-3	-	-	-	-	-	-	-	-	-	-	-	-	**	-	-
C3-4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C3-5	-	-	-	-	-	-	-	-	-	-	-	-	-	-	*
C3-7	-	-	-	-	-	-	-	-	-	-	-	-	-	****	-
C3-8	-	-	-	-	-	-	-	-	*	-	-	-	-	-	***
C3-9	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C14-2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C14-2B	-	-	-	-	-	-	-	-	-	-	-	-	-	***	-
C14-5	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C14-7	-	-	-	-	-	-	-	-	****	****	**	-	-	*	-
C14-8	-	-	-	-	-	-	-	-	-	-	-	-	-	-	*
C14-9	-	-	-	-	-	-	-	-	****	****	-	-	-	-	**
C14-10	-	-	-	-	-	-	-	-	****	****	-	-	-	****	****
C14-12	-	-	-	-	-	-	*	**	***	****	/	/	/	/	/
C14-13	-	-	**	****	****	-	-	-	-	-	-	-	-	-	-
C14-16	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C14-17	-	-	-	-	-	-	-	-	-	-	-	-	-	***	****
C14-18	-	-	****	****	****	-	-	-	-	-	-	-	-	-	-
C14-19	-	-	-	-	-	-	*	-	-	-	-	-	****	****	-
C21-1	-	-	-	-	-	-	-	-	*	****	-	***	****	-	-
C21-2	-	-	-	-	-	-	-	-	-	-	-	****	-	-	-
C21-6	-	-	-	-	-	-	-	-	-	-	/	/	/	/	/
C21-8	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C21-11	-	-	-	-	-	-	-	-	-	-	/	/	/	/	/
C21-13	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C21-17	-	-	-	-	-	-	-	-	-	-	/	/	/	/	/
C21-18	-	-	-	-	-	-	*	***	-	-	-	-	**	***	****
C21-19	-	-	-	-	-	-	-	-	-	-	-	-	-	-	****
C21-20	-	-	-	-	-	-	-	-	-	-	-	-	-	****	****

粗提物编号/浓度 Crude extract No./ Concentration/（µg·mL^-1）	盐度为0.3% Salinity of 0.3%					盐度为3% Salinity of 3%					盐度为10% Salinity of 10%
粗提物编号/浓度 Crude extract No./ Concentration/（µg·mL^-1）	1	10	20	50	100	1	10	20	50	100	1	10	20	50	100
C1-5	-	-	**	****	****	-	-	**	-	-	-	-	*	***	*
C3-1cc	-	-	-	***	-	-	-	-	**	-	-	-	-	****	-
C3-3	-	-	-	****	****	-	-	-	****	****	-	-	**	****	****
C3-4	-	-	-	-	-	-	**	-	-	-	-	**	-	-	-
C3-5	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C3-7	-	-	*	****	****	*	-	-	-	-	-	-	-	-	-
C3-8	-	-	-	-	-	-	****	****	****	-	-	***	****	****	-
C3-9	-	****	****	-	-	*	***	****	****	-	-	-	-	***	****
C14-2	-	-	-	-	-	-	-	***	****	-	/	/	/	/	/
C14-2B	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C14-5	-	****	****	****	-	-	-	-	-	-	-	-	**	-	****
C14-7	/	/	/	/	/	-	-	-	-	-	-	-	-	-	-
C14-8	-	-	-	-	-	-	*	****	****	****	-	-	-	****	-
C14-9	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
C14-10	-	-	-	-	-	-	-	***	****	-	-	-	-	-	-
C14-12	-	-	*	****	**	*	-	-	-	-	-	-	-	-	-
C14-13	-	-	-	*	-	-	-	-	-	-	-	-	****	****	****
C14-16	-	*	-	-	-	-	-	-	-	-	*	**	****	****	-
C14-17	-	***	-	-	-	-	-	-	****	-	-	*	***	****	**
C14-18	-	*	**	-	-	-	**	-	-	-	-	-	-	-	-
C14-19	-	*	-	-	-	-	-	-	-	-	-	-	-	-	-
C21-1	-	-	***	-	-	-	-	-	-	-	-	-	-	****	****
C21-2	-	-	****	-	-	-	-	-	-	-	-	*	****	****	-
C21-6	-	-	-	-	-	*	-	-	-	-	-	-	-	-	***
C21-8	**	-	-	-	-	-	-	-	***	****	-	-	-	-	-
C21-11	-	****	-	-	-	-	*	-	-	-	-	-	-	****	-
C21-13	-	-	-	-	-	**	-	-	-	-	-	-	***	-	-
C21-17	-	-	*	-	-	-	-	****	****	-	*	-	-	****	-
C21-18	-	-	-	-	-	-	-	*	****	-	-	-	-	-	****
C21-19	-	-	-	**	-	-	-	-	-	-	-	-	-	****	**
C21-20	-	-	-	-	-	-	-	-	-	-	-	-	-	*	****

石珊瑚共附生真菌次级代谢产物的抗炎活性及化学多样性研究

Studies on Anti-inflammatory Activity and Chemical Diversity of Secondary Metabolites from Symbiotic Fungi in Stony Corals

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峰编号 Peak No.	化合物名称 Compound name	准分子离子 Quasi-molecular ion	特征碎片离子 Characteristic fragment ions	分子式 Molecular formula	保留时间 Retention time /min	生物来源 Biological origin	峰面积比（3%盐度比10%盐度） Peak area ratio（3% salinity vs 10% salinity）
1	Cordycepin	m/z 274[M+Na]⁺	m/z 136	C₁₀H₁₃N₅O₃	3.143	Plant, Fungi	17.610
2	Visnagin	m/z 231[M+H]⁺	m/z 216, 160, 176	C₁₃H₁₀O₄	3.146	Plant	13.837
3	Thymol	m/z 173[M+Na]⁺	m/z 89, 110, 149	C₁₀H₁₄O	3.216	Plant	12.373
4	6-Methoxyflavone	m/z 275[M+Na]⁺	m/z 238, 210	C₁₆H₁₂O₃	3.888	Plant	11.589
5	Periplogenin	m/z 391[M+H]⁺	m/z 43, 55	C₂₃H₃₄O₅	4.439	Plant	11.486
6	Spathulenol	m/z 243[M+Na]⁺	m/z 202, 205, 43	C₁₅H₂₄O	5.196	Essential oil	11.444
7	Ectoine	m/z 143[M+H]⁺	m/z 143, 97, 101	C₆H₁₀N₂O₂	5.201	Fungi	11.330
8	Gallocatechin	m/z 307[M+H]⁺	m/z 125, 137, 109	C₁₅H₁₄O₇	5.991	Plant	11.165

[1]	张圣良, 楚肖肖, 赵友兴, 孔凡栋, 黄小龙. 海洋生物共附生真菌的分离、鉴定及抗菌活性分析[J]. 生物技术通报, 2019, 35(3): 59-64.
[2]	罗国聪, 黄蕴怡, 柴慧子, 雷晓凌, 聂芳红. 海鞘真菌的形态鉴定及其代谢产物抗菌活性研究[J]. 生物技术通报, 2018, 34(9): 244-248.
[3]	宋恺, 胡洁, 林文翰, 季宇彬. 海绵共附生真菌多样性及其次级代谢产物的研究进展[J]. 生物技术通报, 2014, 0(4): 36-42.