基于重测序的菠萝基因组InDel标记的开发

doi:10.13560/j.cnki.biotech.bull.1985.2024-0728

摘要/Abstract

摘要：

目的基于菠萝种质的基因组重测序数据研发菠萝InDel分子标记，为菠萝种质资源的创新利用奠定理论基础。方法选取4份代表性菠萝种质进行基因组重测序，鉴定插入/缺失长度大于30 bp的InDel位点。根据InDel位点信息设计InDel标记引物，选择扩增条带清晰、分离效果好的引物用于菠萝种质遗传多样性分析。结果在4份菠萝种质中鉴定到插入/缺失长度大于30 bp、测序深度大于10 ×的InDel位点1 559个。基于InDel位点的上下游序列信息，在全基因组范围内设计候选InDel标记引物372对，其中264对引物表现出稳定的多态性，多态性比例为70.97%。采用50对多态性高的核心标记引物分析58份菠萝种质的遗传多样性。共检测到等位位点103个；各标记的平均有效等位位点数（Ne）为1.816 4个；Shannon’s多样性指数（I）的变异范围为0.272 8-0.746 4，平均值为0.634 6；多态性信息含量（PIC）为0.132 9-0.425 1，平均值为0.342 2；Nei’s基因多样性指数（H）变异范围为0.143 1-0.514 3，平均值为0.441 2。通过聚类分析在遗传距离0.75处将58份菠萝种质分为4个类群，其中第Ⅳ类群可进一步分为2个亚群。采用群体结构分析将58份种质划分为2个稳定群体，其中POP-1群体与聚类分析获得的第Ⅳ-2亚群重合，主要由纯种卡因类种质组成。结论研发的50个菠萝核心InDel标记能精准区分不同菠萝品种，对于深化菠萝遗传多样性分析、完善遗传图谱构建和加速分子标记辅助育种进程具有重要意义。

关键词: 菠萝, 重测序, InDel标记, 遗传多样性分析

Abstract:

Objective The goal is to develop InDel molecular markers based on genome re-sequencing data, laying a theoretical foundation for innovative utilization of pineapple resources. Method Four representative pineapple accessions were selected for genome re-sequencing to identify InDel loci with insertion/deletion lengths > 30 bp. The InDel marker primers were designed based on the sequence information of these InDel loci, and those having clear amplification bands and favorable separation effects were selected for further analysis of genetic diversity. Result The 1 559 InDel loci with insertion/deletion length >30 bp and sequencing depths > 10 × were identified from re-sequencing data of four pineapple accessions. Based on the upstream and downstream sequences of these InDel loci, 372 pairs of InDel marker primers were designed across the entire genome, of which 264 pairs showed stable polymorphism, with a polymorphism ratio of 70.97%. The 50 of the 264 polymorphic InDel markers were assigned as core markers, further used for the genetic diversity analyzing of 58 pineapple accessions. A total of 103 alleles were identified, and the average effective numbers of alleles (Ne) for each marker are 1.816 5. The Shannon's Information index (I) ranged from 0.272 8 to 0.746 4, with an average value of 0.634 6. The polymorphism information content (PIC) valued from 0.132 9 to 0.425 1, with an average value of 0.342 2. While the variation range of Nei's gene diversity index (H) was 0.143 1 to 0.514 3, with an average value of 0.441 2. Through cluster analysis, 58 pineapple accessions were classified into 4 groups at a genetic distance of 0.75, with group IV can be further divided into 2 subgroups. The 58 pineapple accessions can also be divided into two stable populations through structure analysis, with the POP-1 population overlapping with the Ⅳ-2 subgroup obtained from cluster analysis, mainly composed of smooth cayenne germplasms. Conclusion The 50 core InDel markers developed in this study can accurately distinguish different pineapple accessions, which helps for deepening the analysis of pineapple genetic diversity, improving the construction of genetic map, and accelerating the process of molecular marker assisted breeding.

Key words: pineapple, re-sequencing, InDel markers, genetic diversity analysis

贺涵, 刘传和, 喻梦凡, 袁梦萍, 魏岳荣, 杨敏, 邝瑞彬, 周陈平, 吴夏明, 徐泽. 基于重测序的菠萝基因组InDel标记的开发[J]. 生物技术通报, 2025, 41(2): 65-76.

HE Han, LIU Chuan-he, YU Meng-fan, YUAN Meng-ping, WEI Yue-rong, YANG Min, KUANG Rui-bin, ZHOU Chen-ping, WU Xia-ming, XU Ze. Development of Insertion-deletion Markers in Ananas comosus of Genome Based on Re-sequencing Data[J]. Biotechnology Bulletin, 2025, 41(2): 65-76.

图/表 6

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标记编号 InDel tag	染色体位置 Location	上游引物 Forward primer	上游引物序列 Forward primer sequence（5'-3'）	下游引物 Reverse primer	下游引物序列 Reverse primer sequence（5'-3'）
P1-3	Chr1， 12031084	P1-3-F	TGGCTTCATGCTTCTTTTGCA	P1-3-R	TCAGACGCAGCCTGGTTG
P1-13	Chr1， 23303433	P1-13-F	GACTGCGCTCGGAAGTGA	P1-13-R	AGAAGGAGGAGGAAGGGGG
P2-1	Chr2， 2165847	P2-1-F	GCGGAGTGAACGATCCCT	P2-1-R	AGGAAAGTTTGGGTGCGTCT
P2-8	Chr2， 13525182	P2-8-F	TGGCAACATTCCCCTATGCA	P2-8-R	GGGGTCCTAAAGGGTCAAGC
P3-1	Chr3， 844091	P3-1-F	CCGAGAAAAGCGACAGCC	P3-1-R	TGCAGCGCAACTAAGAGGT
P3-4	Chr3， 1725841	P3-4-F	CCACAGGCCCCTCCATTG	P3-4-R	CCGCCAGACTGCTTTCGA
P4-4	Chr4， 5231326	P4-4-F	GGTCGAAGGCGCACTGTA	P4-4-R	CATGCCCGGCGAAGGATA
P4-9	Chr4， 13043220	P4-9-F	AGGAAAGAAGCAACAAGGCCT	P4-9-R	CCAAGGGGTGCGAACGTA
P5-7	Chr5， 1511655	P5-7-F	CTGCAAGGTGCTTCATTGAGT	P5-7-R	GGCATCCCTTGTCCAGGT
P5-9	Chr5， 2289873	P5-9-F	GGAATCCACAACCCCATTGC	P5-9-R	CAAGGGGTTGTGAAACTGCA
P6-4	Chr6， 10732786	P6-4-F	CCTGCGGGAGGATTCAGG	P6-4-R	GGCGGAATAAGGGCCTCG
P6-12	Chr6， 3392699	P6-12-F	GCAGCCAGCAGAACAACA	P6-12-R	GGCTTGAAGAACCCATCGC
P7-3	Chr7， 12588489	P7-3-F	GAAGCCCGACACGACCAG	P7-3-R	TGACCAGCACGAACGACC
P7-13	Chr7， 12954807	P7-13-F	CCCTTTGTCCTACAAATGGGG	P7-13-R	TCGCATGAACAGTGAGGCA
P8-2	Chr8， 9924768	P8-2-F	CGCCCACGCTCCTAAGAG	P8-2-R	GCACCATTCAACAAGCCACA
P8-4	Chr8， 12420683	P8-4-F	CGGTGAATTTGCTACACGGT	P8-4-R	TGTGGTAGCATAGATTGGCGA
P9-8	Chr9， 11439477	P9-8-F	CGGTGTATCGAGGTCGCC	P9-8-R	CCAGCGCCTTGAAGTCCA
P9-13	Chr9， 12410107	P9-13-F	AGTTCATTTGAAGTGGTGCCT	P9-13-R	TGTTTTGGGCCGATGAAAGA
P10-7	Chr10， 82948	P10-7-F	GGGCACAGCGACGTTACA	P10-7-R	GGGCCTGCCGTACACTTT
P10-15	Chr10， 3770865	P10-15-F	AAGATGTTTGGCCGTGCG	P10-15-R	CGCGCTGATGAGGAAAGC
P11-12	Chr11， 3139788	P11-12-F	TCAGATGCCCGAGGTCCA	P11-12-R	TGACATCCCGAATGCGCA
P11-15	Chr11， 9182497	P11-15-F	GTGGACGACAATGGCGGA	P11-15-R	TCTCAGCAACCATGTCGCA
P12-3	Chr12， 11355510	P12-3-F	AGGAGGCTTCGGAGTCGA	P12-3-R	CTGGGGTGTCACACTATCTGA
P12-7	Chr12， 3380797	P12-7-F	AGAAACCAAGGGCTCCGA	P12-7-R	TCGATCCGCATCACACGT
P13-5	Chr13， 1779898	P13-5-F	CACCGGGGCACGTCTAAA	P13-5-R	GGGCTTTGTGATTTTTGGGCT
P13-9	Chr13， 3803304	P13-9-F	GGGCATGCCGACCGAATA	P13-9-R	TGGTGCCCACTTGGTGAC
P14-2	Chr14， 1157124	P14-2-F	CTTCTTTGGCGGGGAGGG	P14-2-R	GTATGCAGTGGCCACCGT
P14-15	Chr14， 9737746	P14-15-F	TGGTTGCGTTGGTTGGGT	P14-15-R	CTCTGGAAGCCCAATCTCGA
P15-11	Chr15， 6253581	P15-11-F	GGGCACCGGACCAACTTT	P15-11-R	GGACGGCTAACAGGTTTCGA
P15-12	Chr15， 7964660	P15-12-F	GTGCCGTACCGTGCTAGG	P15-12-R	CTGGGCAGTTGGATCTTGGT
P16-6	Chr16， 1035082	P16-6-F	GTCGCGATTTCCCCACCA	P16-6-R	GCGTTTGATTGCTCAGCCA
P16-13	Chr16， 11032414	P16-13-F	CTGTACCTGTCGGTGGCG	P16-13-R	TGGCAGTTCAGCGACCAG
P17-7	Chr17， 2630836	P17-7-F	GCGCCGTAGTAGAGTTCCA	P17-7-R	TTCCCGTGTCACAGGCAC
P17-11	Chr17， 8060219	P17-11-F	TGAATGGGAAAGCAACACGC	P17-11-R	GCATCTACCGCACCCTTGA
P18-1	Chr18， 4131951	P18-1-F	CAGCGAGAGAGCGATGCA	P18-1-R	CAAGGGCGGTTGGTGGAT
P18-13	Chr18， 5367546	P18-13-F	TGCAGTCGCATATGCAGCT	P18-13-R	ACTCTGGCGGGAAGTCGA
P19-1	Chr19， 8770836	P19-1-F	CCGAAACAGCAACTCAAATGC	P19-1-R	GCCTCACCTTGTGGTTGC
P19-7	Chr19， 9071773	P19-7-F	GAACGGGAGGAGCGATCG	P19-7-R	TCCTCGTATCACCGATCCCT
P20-1	Chr20， 4990506	P20-1-F	TGAATGCCAATCCTTGCACC	P20-1-R	CTGAGAGCATCACAGCTTGTG
P20-9	Chr20， 7316522	P20-9-F	TGCAGGCAATGATGGAGCA	P20-9-R	CACCTTAATGAAGCCGCAGC
P21-3	Chr21， 10154987	P21-3-F	TCGGGTTTTGACAAGGGAGT	P21-3-R	GAGTTTCTCATTGAGTGGGCA
P21-8	Chr21， 8270960	P21-8-F	TGCTGAGCTAAACCTTAGGCA	P21-8-R	CGTGGGTCAATGCTTTCCC
P22-14	Chr22， 73132	P22-14-F	GCGCAATCTCTTCCGACC	P22-14-R	GCAACCACAGCAGCCAAC
P22-15	Chr22， 1287004	P22-15-F	TGGCAATGAGTTGTGATTGGT	P22-15-R	TCGGAAAAACACTGAGCTGA
P23-7	Chr23， 3066097	P23-7-F	CGAACGGTGAAACAGGGGA	P23-7-R	GCAGGTAGTAAAGTTGCTCGC
P23-15	Chr23， 3085762	P23-15-F	ACTAGAACGAGTCCGGGCA	P23-15-R	AGCGCCAGTCGAAAGAACA
P24-7	Chr24， 40948	P24-7-F	GGGTCCCGACGTAGGAGT	P24-7-R	ACGAGATCTGGTCCGGCT
P24-8	Chr24， 6182284	P24-8-F	CGGGGGAGTATGTGACACC	P24-8-R	GGTTGATGCAGCACGCTG
P25-5	Chr25， 2263248	P25-5-F	GCACATTTCACTTCGAGTCGA	P25-5-R	GAAGGTGGAATGGACCCCA
P25-10	Chr25， 2380378	P25-10-F	CTCGACAACTGCTGCGGA	P25-10-R	AGGTGGATGAGACACGTAACT

标记编号 InDel tag	染色体位置 Location	上游引物 Forward primer	上游引物序列 Forward primer sequence（5'-3'）	下游引物 Reverse primer	下游引物序列 Reverse primer sequence（5'-3'）
P1-3	Chr1， 12031084	P1-3-F	TGGCTTCATGCTTCTTTTGCA	P1-3-R	TCAGACGCAGCCTGGTTG
P1-13	Chr1， 23303433	P1-13-F	GACTGCGCTCGGAAGTGA	P1-13-R	AGAAGGAGGAGGAAGGGGG
P2-1	Chr2， 2165847	P2-1-F	GCGGAGTGAACGATCCCT	P2-1-R	AGGAAAGTTTGGGTGCGTCT
P2-8	Chr2， 13525182	P2-8-F	TGGCAACATTCCCCTATGCA	P2-8-R	GGGGTCCTAAAGGGTCAAGC
P3-1	Chr3， 844091	P3-1-F	CCGAGAAAAGCGACAGCC	P3-1-R	TGCAGCGCAACTAAGAGGT
P3-4	Chr3， 1725841	P3-4-F	CCACAGGCCCCTCCATTG	P3-4-R	CCGCCAGACTGCTTTCGA
P4-4	Chr4， 5231326	P4-4-F	GGTCGAAGGCGCACTGTA	P4-4-R	CATGCCCGGCGAAGGATA
P4-9	Chr4， 13043220	P4-9-F	AGGAAAGAAGCAACAAGGCCT	P4-9-R	CCAAGGGGTGCGAACGTA
P5-7	Chr5， 1511655	P5-7-F	CTGCAAGGTGCTTCATTGAGT	P5-7-R	GGCATCCCTTGTCCAGGT
P5-9	Chr5， 2289873	P5-9-F	GGAATCCACAACCCCATTGC	P5-9-R	CAAGGGGTTGTGAAACTGCA
P6-4	Chr6， 10732786	P6-4-F	CCTGCGGGAGGATTCAGG	P6-4-R	GGCGGAATAAGGGCCTCG
P6-12	Chr6， 3392699	P6-12-F	GCAGCCAGCAGAACAACA	P6-12-R	GGCTTGAAGAACCCATCGC
P7-3	Chr7， 12588489	P7-3-F	GAAGCCCGACACGACCAG	P7-3-R	TGACCAGCACGAACGACC
P7-13	Chr7， 12954807	P7-13-F	CCCTTTGTCCTACAAATGGGG	P7-13-R	TCGCATGAACAGTGAGGCA
P8-2	Chr8， 9924768	P8-2-F	CGCCCACGCTCCTAAGAG	P8-2-R	GCACCATTCAACAAGCCACA
P8-4	Chr8， 12420683	P8-4-F	CGGTGAATTTGCTACACGGT	P8-4-R	TGTGGTAGCATAGATTGGCGA
P9-8	Chr9， 11439477	P9-8-F	CGGTGTATCGAGGTCGCC	P9-8-R	CCAGCGCCTTGAAGTCCA
P9-13	Chr9， 12410107	P9-13-F	AGTTCATTTGAAGTGGTGCCT	P9-13-R	TGTTTTGGGCCGATGAAAGA
P10-7	Chr10， 82948	P10-7-F	GGGCACAGCGACGTTACA	P10-7-R	GGGCCTGCCGTACACTTT
P10-15	Chr10， 3770865	P10-15-F	AAGATGTTTGGCCGTGCG	P10-15-R	CGCGCTGATGAGGAAAGC
P11-12	Chr11， 3139788	P11-12-F	TCAGATGCCCGAGGTCCA	P11-12-R	TGACATCCCGAATGCGCA
P11-15	Chr11， 9182497	P11-15-F	GTGGACGACAATGGCGGA	P11-15-R	TCTCAGCAACCATGTCGCA
P12-3	Chr12， 11355510	P12-3-F	AGGAGGCTTCGGAGTCGA	P12-3-R	CTGGGGTGTCACACTATCTGA
P12-7	Chr12， 3380797	P12-7-F	AGAAACCAAGGGCTCCGA	P12-7-R	TCGATCCGCATCACACGT
P13-5	Chr13， 1779898	P13-5-F	CACCGGGGCACGTCTAAA	P13-5-R	GGGCTTTGTGATTTTTGGGCT
P13-9	Chr13， 3803304	P13-9-F	GGGCATGCCGACCGAATA	P13-9-R	TGGTGCCCACTTGGTGAC
P14-2	Chr14， 1157124	P14-2-F	CTTCTTTGGCGGGGAGGG	P14-2-R	GTATGCAGTGGCCACCGT
P14-15	Chr14， 9737746	P14-15-F	TGGTTGCGTTGGTTGGGT	P14-15-R	CTCTGGAAGCCCAATCTCGA
P15-11	Chr15， 6253581	P15-11-F	GGGCACCGGACCAACTTT	P15-11-R	GGACGGCTAACAGGTTTCGA
P15-12	Chr15， 7964660	P15-12-F	GTGCCGTACCGTGCTAGG	P15-12-R	CTGGGCAGTTGGATCTTGGT
P16-6	Chr16， 1035082	P16-6-F	GTCGCGATTTCCCCACCA	P16-6-R	GCGTTTGATTGCTCAGCCA
P16-13	Chr16， 11032414	P16-13-F	CTGTACCTGTCGGTGGCG	P16-13-R	TGGCAGTTCAGCGACCAG
P17-7	Chr17， 2630836	P17-7-F	GCGCCGTAGTAGAGTTCCA	P17-7-R	TTCCCGTGTCACAGGCAC
P17-11	Chr17， 8060219	P17-11-F	TGAATGGGAAAGCAACACGC	P17-11-R	GCATCTACCGCACCCTTGA
P18-1	Chr18， 4131951	P18-1-F	CAGCGAGAGAGCGATGCA	P18-1-R	CAAGGGCGGTTGGTGGAT
P18-13	Chr18， 5367546	P18-13-F	TGCAGTCGCATATGCAGCT	P18-13-R	ACTCTGGCGGGAAGTCGA
P19-1	Chr19， 8770836	P19-1-F	CCGAAACAGCAACTCAAATGC	P19-1-R	GCCTCACCTTGTGGTTGC
P19-7	Chr19， 9071773	P19-7-F	GAACGGGAGGAGCGATCG	P19-7-R	TCCTCGTATCACCGATCCCT
P20-1	Chr20， 4990506	P20-1-F	TGAATGCCAATCCTTGCACC	P20-1-R	CTGAGAGCATCACAGCTTGTG
P20-9	Chr20， 7316522	P20-9-F	TGCAGGCAATGATGGAGCA	P20-9-R	CACCTTAATGAAGCCGCAGC
P21-3	Chr21， 10154987	P21-3-F	TCGGGTTTTGACAAGGGAGT	P21-3-R	GAGTTTCTCATTGAGTGGGCA
P21-8	Chr21， 8270960	P21-8-F	TGCTGAGCTAAACCTTAGGCA	P21-8-R	CGTGGGTCAATGCTTTCCC
P22-14	Chr22， 73132	P22-14-F	GCGCAATCTCTTCCGACC	P22-14-R	GCAACCACAGCAGCCAAC
P22-15	Chr22， 1287004	P22-15-F	TGGCAATGAGTTGTGATTGGT	P22-15-R	TCGGAAAAACACTGAGCTGA
P23-7	Chr23， 3066097	P23-7-F	CGAACGGTGAAACAGGGGA	P23-7-R	GCAGGTAGTAAAGTTGCTCGC
P23-15	Chr23， 3085762	P23-15-F	ACTAGAACGAGTCCGGGCA	P23-15-R	AGCGCCAGTCGAAAGAACA
P24-7	Chr24， 40948	P24-7-F	GGGTCCCGACGTAGGAGT	P24-7-R	ACGAGATCTGGTCCGGCT
P24-8	Chr24， 6182284	P24-8-F	CGGGGGAGTATGTGACACC	P24-8-R	GGTTGATGCAGCACGCTG
P25-5	Chr25， 2263248	P25-5-F	GCACATTTCACTTCGAGTCGA	P25-5-R	GAAGGTGGAATGGACCCCA
P25-10	Chr25， 2380378	P25-10-F	CTCGACAACTGCTGCGGA	P25-10-R	AGGTGGATGAGACACGTAACT

标记编号 InDel tag	有效等位位点数（Ne） Effective number of alleles	Shannon's多样性指数（I） Shannon's information index	多态性信息含量（PIC） Polymorphic information content	Nei's基因多样性指数（H） Nei's gene diversity index
P1-3	1.999 4	0.693 0	0.374 9	0.499 9
P1-13	1.167 0	0.272 8	0.132 9	0.143 1
P2-1	1.768 2	0.626 1	0.340 1	0.434 5
P2-8	1.806 2	0.638 5	0.346 7	0.446 3
P3-1	1.555 6	0.542 6	0.293 4	0.357 2
P3-4	1.904 3	0.667 8	0.362 1	0.474 9
P4-4	1.768 2	0.626 1	0.340 1	0.434 5
P4-9	1.686 6	0.597 1	0.324 2	0.407 1
P5-7	1.978 8	0.687 8	0.372 3	0.494 6
P5-9	1.806 2	0.638 5	0.346 7	0.446 3
P6-4	1.917 9	0.671 6	0.364 1	0.478 6
P6-12	1.622 0	0.571 6	0.309 9	0.383 5
P7-3	2.000 0	0.693 1	0.375 0	0.500 0
P7-13	1.999 4	0.693 0	0.374 9	0.499 9
P8-2	1.904 3	0.667 8	0.362 1	0.474 9
P8-4	1.858 6	0.654 6	0.355 3	0.462 0
P9-8	1.997 6	0.692 6	0.374 7	0.499 4
P9-13	1.858 6	0.654 6	0.355 3	0.462 0
P10-7	2.058 8	0.825 1	0.425 1	0.514 3
P10-15	1.768 2	0.626 1	0.340 1	0.434 5
P11-12	1.999 4	0.693 0	0.374 9	0.499 9
P11-15	1.622 0	0.571 6	0.309 9	0.383 5
P12-3	1.997 6	0.692 6	0.374 7	0.499 4
P12-7	1.787 5	0.632 5	0.343 5	0.440 5
P13-5	1.421 5	0.473 0	0.252 6	0.296 5
P13-9	1.686 6	0.597 1	0.324 2	0.407 1
P14-2	1.643 8	0.580 5	0.315 0	0.391 6
P14-15	1.978 8	0.687 8	0.372 3	0.494 6
P15-11	1.990 5	0.690 8	0.373 8	0.497 6
P15-12	1.999 4	0.693 0	0.374 9	0.499 9
P16-6	1.962 7	0.683 6	0.370 2	0.490 5
P16-13	1.600 0	0.562 3	0.304 7	0.375 0
P17-7	1.169 9	0.313 9	0.138 5	0.145 2
P17-11	1.942 3	0.678 2	0.367 5	0.485 1
P18-1	1.874 6	0.659 3	0.357 7	0.466 6
P18-13	1.748 4	0.619 4	0.336 4	0.428 1
P19-1	1.824 3	0.644 2	0.349 8	0.451 8
P19-7	1.841 8	0.649 6	0.352 6	0.457 0
P20-1	1.889 9	0.663 7	0.360 0	0.470 9
P20-9	1.889 9	0.663 7	0.360 0	0.470 9
P21-3	1.577 9	0.552 7	0.299 2	0.366 2
P21-8	1.904 3	0.667 8	0.362 1	0.474 9
P22-14	1.917 9	0.671 6	0.364 1	0.478 6
P22-15	1.990 5	0.690 8	0.373 8	0.497 6
P23-7	1.643 8	0.580 5	0.315 0	0.391 6
P23-15	1.665 3	0.589 0	0.319 7	0.399 5
P24-7	1.981 7	0.746 4	0.388 6	0.495 4
P24-8	1.904 3	0.667 8	0.362 1	0.474 9
P25-5	1.997 6	0.692 6	0.374 7	0.499 4
P25-10	1.942 3	0.678 2	0.367 5	0.485 1
平均值	1.816 4	0.634 6	0.342 2	0.441 2

标记编号 InDel tag	有效等位位点数（Ne） Effective number of alleles	Shannon's多样性指数（I） Shannon's information index	多态性信息含量（PIC） Polymorphic information content	Nei's基因多样性指数（H） Nei's gene diversity index
P1-3	1.999 4	0.693 0	0.374 9	0.499 9
P1-13	1.167 0	0.272 8	0.132 9	0.143 1
P2-1	1.768 2	0.626 1	0.340 1	0.434 5
P2-8	1.806 2	0.638 5	0.346 7	0.446 3
P3-1	1.555 6	0.542 6	0.293 4	0.357 2
P3-4	1.904 3	0.667 8	0.362 1	0.474 9
P4-4	1.768 2	0.626 1	0.340 1	0.434 5
P4-9	1.686 6	0.597 1	0.324 2	0.407 1
P5-7	1.978 8	0.687 8	0.372 3	0.494 6
P5-9	1.806 2	0.638 5	0.346 7	0.446 3
P6-4	1.917 9	0.671 6	0.364 1	0.478 6
P6-12	1.622 0	0.571 6	0.309 9	0.383 5
P7-3	2.000 0	0.693 1	0.375 0	0.500 0
P7-13	1.999 4	0.693 0	0.374 9	0.499 9
P8-2	1.904 3	0.667 8	0.362 1	0.474 9
P8-4	1.858 6	0.654 6	0.355 3	0.462 0
P9-8	1.997 6	0.692 6	0.374 7	0.499 4
P9-13	1.858 6	0.654 6	0.355 3	0.462 0
P10-7	2.058 8	0.825 1	0.425 1	0.514 3
P10-15	1.768 2	0.626 1	0.340 1	0.434 5
P11-12	1.999 4	0.693 0	0.374 9	0.499 9
P11-15	1.622 0	0.571 6	0.309 9	0.383 5
P12-3	1.997 6	0.692 6	0.374 7	0.499 4
P12-7	1.787 5	0.632 5	0.343 5	0.440 5
P13-5	1.421 5	0.473 0	0.252 6	0.296 5
P13-9	1.686 6	0.597 1	0.324 2	0.407 1
P14-2	1.643 8	0.580 5	0.315 0	0.391 6
P14-15	1.978 8	0.687 8	0.372 3	0.494 6
P15-11	1.990 5	0.690 8	0.373 8	0.497 6
P15-12	1.999 4	0.693 0	0.374 9	0.499 9
P16-6	1.962 7	0.683 6	0.370 2	0.490 5
P16-13	1.600 0	0.562 3	0.304 7	0.375 0
P17-7	1.169 9	0.313 9	0.138 5	0.145 2
P17-11	1.942 3	0.678 2	0.367 5	0.485 1
P18-1	1.874 6	0.659 3	0.357 7	0.466 6
P18-13	1.748 4	0.619 4	0.336 4	0.428 1
P19-1	1.824 3	0.644 2	0.349 8	0.451 8
P19-7	1.841 8	0.649 6	0.352 6	0.457 0
P20-1	1.889 9	0.663 7	0.360 0	0.470 9
P20-9	1.889 9	0.663 7	0.360 0	0.470 9
P21-3	1.577 9	0.552 7	0.299 2	0.366 2
P21-8	1.904 3	0.667 8	0.362 1	0.474 9
P22-14	1.917 9	0.671 6	0.364 1	0.478 6
P22-15	1.990 5	0.690 8	0.373 8	0.497 6
P23-7	1.643 8	0.580 5	0.315 0	0.391 6
P23-15	1.665 3	0.589 0	0.319 7	0.399 5
P24-7	1.981 7	0.746 4	0.388 6	0.495 4
P24-8	1.904 3	0.667 8	0.362 1	0.474 9
P25-5	1.997 6	0.692 6	0.374 7	0.499 4
P25-10	1.942 3	0.678 2	0.367 5	0.485 1
平均值	1.816 4	0.634 6	0.342 2	0.441 2

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