无筛选标记转基因作物的研究进展

摘要/Abstract

摘要： 目前，大部分植物遗传转化都要使用选择标记基因，诸如抗生素、除草剂等来筛选转化子，由此而引起的转基因生物安全性问题受到了人们的关注。为了消除转基因作物中的筛选标记，一种全新的策略即无选择标记的转基因技术迅速发展起来。该技术既能够减轻大众对转基因植物中含有选择标记基因而引起的恐惧，又能将多个基因逐个整合到同一作物以实现基因聚合。因此这种新策略有着巨大的应用价值。对获得无筛选标记转基因作物的一些方法及其最新研究进展进行综述。

关键词: 筛选标记, 位点特异性重组, 转座子

Abstract: Selectable marker genes（SMGs）based on antibiotic or herbicide resistance have been widely used to identify ‘true’transformants. To release the public concerns for the bio-safety of SMGs，a new strategy has been developed to obtain marker-free transgenicplants to eliminate the marker gene from the nuclear or chloroplast genome after selection. Marker-free transgenic crops have a lot of uniqueadvantage such as reducing the trepidations from the SMGs and transferring exogenous genes gradually into transgenic crops. In this review，different approaches of marker-free transgenic method were discussed.

Key words: Selectable marker genes（SMGs）, Site-specific recombination, Transposons

陈扬勋, 张治国, 路铁刚. 无筛选标记转基因作物的研究进展[J]. 生物技术通报, 2012, 0(12): 1-7.

Chen Yangxun, Zhang Zhiguo, Lu Tiegang. Review of Marker-free Transgenic Crop[J]. Biotechnology Bulletin, 2012, 0(12): 1-7.

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