幽门螺杆菌尿素酶A基因克隆表达及动物抗血清免疫保护作用研究

生物技术通报 ›› 2013, Vol. 0 ›› Issue (8): 135-138.

幽门螺杆菌尿素酶A基因克隆表达及动物抗血清免疫保护作用研究

刘文¹, 汤艳超¹, 黄睿², 崔海燕¹, 袁金山¹, 胡巍¹

（1.山东理工大学生命科学学院，淄博 255049；2.河南中医学院基础医学院，郑州 450008）

收稿日期:2013-01-30 修回日期:2013-08-11 出版日期:2013-08-11 发布日期:2013-09-02
作者简介:通讯作者：胡巍，男，博士，副教授，研究方向：微生物分子免疫学；E-mail：sdhuwei@sdut.edu.cn
基金资助:
作者简介：刘文，女，硕士，副教授，研究方向：微生物分子生物学；E-mail：zblwen@163.com

Cloning and Expression of Helicobacter pylori Urease Subunit A and Its Immune Cross Protection Between Animal Antiserum and Patient Serum

Liu Wen¹, Tang Yanchao¹, Huang Rui², Cui Haiyan¹, Yuan Jinshan¹, Hu Wei¹

（1. School of Life Sciences，Shandong University of Technology，Zibo 255049；2. School of Basic Medicine，Henan College of Traditional Chinese Medicine, Zhengzhou 450008）

Received:2013-01-30 Revised:2013-08-11 Published:2013-08-11 Online:2013-09-02

摘要/Abstract

摘要： 旨在研究动物抗血清在抗幽门螺杆菌感染方面的免疫交叉保护作用。以幽门螺杆菌标准株基因组为模板，PCR特异性扩增尿素酶A亚基基因，构建表达载体pBVIL1-ureA并转化大肠杆菌，诱导转化菌表达，用纯化的表达蛋白免疫家兔得到家兔抗血清，用抗血清和病人血清进行ELISA试验。结果显示，原核表达的抗原蛋白IL1-UreA既能与兔抗血清反应也能与幽门螺杆菌感染的病人血清反应，且两种血清与抗原蛋白存在竞争性结合反应。动物抗血清具有免疫保护作用，可以利用动物特异性抗体进行幽门螺杆菌感染的免疫预防和免疫治疗。

关键词: 幽门螺杆菌, 尿素酶A亚基, 抗血清, 免疫保护

Abstract: In this paper, immune cross protection from ureA antigen between rabbit antiserum and patient serum, infected by H. pylori, was tested. The ureA gene from H. pylori 26695 strain was amplified with PCR technology and cloned into expressive vector pBVIL1 and the recombinant pBVIL1-ureA was transformed into DH5α strain. The ureA antigen purified was used to immune rabbit three times and antiserum was acquired. The antiserum titre and immune cross protection were tested with ELISA. The results showed that the antigen expressed by prokaryotic system could specifically react on both patient serum and rabbit antiserum and possess a competitive immune cross reaction. As a result, it was concluded that animal antibodies against H. pylori may be applied in immune precaution and therapy of infections diseases from H. pylori.

Key words: Helicobacter pylori, Urease subunit A, Antiserum, Immune protection

刘文, 汤艳超, 黄睿, 崔海燕, 袁金山, 胡巍. 幽门螺杆菌尿素酶A基因克隆表达及动物抗血清免疫保护作用研究[J]. 生物技术通报, 2013, 0(8): 135-138.

Liu Wen, Tang Yanchao, Huang Rui, Cui Haiyan, Yuan Jinshan, Hu Wei. Cloning and Expression of Helicobacter pylori Urease Subunit A and Its Immune Cross Protection Between Animal Antiserum and Patient Serum[J]. Biotechnology Bulletin, 2013, 0(8): 135-138.

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