Comparison on Muscle Growth and Expression of Muscle Growth- related Genes Among Three Mandarin Fishes

doi:10.13560/j.cnki.biotech.bull.1985.2014.12.031

Abstract

Abstract: Muscle samples of Siniperca chuatsi ♂×S. scherzeri ♀ F1 and their parents at five developmental stages in 90 days after hatching. The skeletal muscle growth characteristics were identified by numbers and diameters of muscle fibers through paraffin sections.Meantime were obtained and the mRNA expression of myostatin, MyoD, myogenin in muscle was detected by quantitative PCR. The results showed there were significant differences in growth among three mandarin fish. Siniperca chautsi grew the fastest, F1 secondly and S. scherzeri kept the last. At different developmental stages, numbers of muscle fibers in three mandarin fish showed no significant difference, while the average diameters of the muscle fibers showed positive correlation with growth rate. Growth differences among three mandarin were mainly caused by muscle hypertrophy. The myostatin mRNA expression peak appeared in D20, then the expression showed a downward trend. MyoD mRNA expressed highly in D10, D20 and decreased in following days. The myogenin mRNA expression was the lowest in D20, followed by an obviously increase in Siniperca chuatsi, and F1 secondly, while S. scherzeri stable. It showed correlations between muscle growth and gene expression in three mandarin at different growth stages.

Key words: Siniperca, Growth, Muscle fiber, Myostatin, MyoD, Myogenin, mRNA expression

Xu Miaoyang,Zhao Jinliang,Li Chuanyang,Qian Yezhou,Wu Chao,Qian De. Comparison on Muscle Growth and Expression of Muscle Growth- related Genes Among Three Mandarin Fishes[J]. Biotechnology Bulletin, 2014, 0(12): 184-189.

References

[1] 梁旭方. 国内外鳜类研究及养殖概况[J]. 水产科技情报, 1996, 23(1)：13-17.
[2] 黄志坚, 何建国. 鳜鱼疾病的研究概况[J]. 水产科技情报, 1999, 26(6)：268-271.
[3] 许建红, 劳顺健. 斑鳜人工养殖初探[J]. 科学养鱼, 2002, 7: 26.
[4] 楼允东. 鱼类育种学[M]. 北京: 中国农业出版社, 1999.
[5] 楼允东, 李小勤. 中国鱼类远缘杂交研究及其在水产养殖上的应用[J]. 中国水产科学, 2006, 13(1)：151-158.
[6] 钱叶周, 吴超, 钱德, 等. 斑鳜 × 鳜杂交一代与选育一代网箱养殖生长比较[J]. 水产养殖, 2013, 32(7)：1-4.
[7] S?nger AM, Stoiber W. Muscle fiber diversity and plasticity[J]. Fish Physiology, 2001, 18: 187-250.
[8] 成嘉, 褚武英, 张建社. 鱼类肌肉组织发生和分化相关基因的研究进展[J]. 生命科学研究, 2010, 14(4)：355-362.
[9] 苌建菊, 赵金良, 苗田田. 鳜慢肌胚后发育与生长特征[J]. 动物学杂志, 2012, 47(4)：89-95.
[10] Rowlerson A. Veggetti A. Cellular mechanisms of post-embryonic muscle growth in aquaculture species[J]. Fish Physiology, 2001, 18: 103-140.
[11] Buckingham M. Molecular biology of muscle development[J]. Cell, 1994, 78(1)：15-21.
[12] 刘丑生, 赵兴波, 李宁, 等. 动物肌肉生长发育调控的功能基因研究进展[J]. 中国畜牧杂志, 2003, 39(5)：48-49.
[13] 李永平, 梁炳生. MyoD 肌形成作用机制研究进展[J]. 国际骨科学杂志, 2007, 28(1)：37-40.
[14] 邱华玲, 于建兴, 陈宏权. MyoG 基因结构与功能研究进展[J]. 生物信息学, 2006, 4: 190-192.
[15] 邵芳, 卢祥云, 顾志良. 鱼类 MSTN 基因的研究进展[J]. 常熟理工学院学报: 自然科学版, 2011, 25(8)：76-80.
[16] 赵浩斌, 彭扣, 王玉凤, 等. 鱼类肌肉生长抑制素研究进展[J]. 水生生物学报, 2006, 30(2)：227-231.
[17] 许淼洋, 郭金涛, 赵金良, 等. 斑鳜(♀)× 鳜(♂)杂交子一代、子二代的形态特征分析[J]. 水产科学, 2013, 32(11)： 636-640.
[18] 李传阳, 许淼洋, 等. 斑鳜(♀)× 鳜(♂)杂交后代遗传特征的微卫星分析[J]. 水产科学, 2014, 33(2)：97-102.
[19] 邵芳, 卢祥云, 顾志良. 鱼类 MSTN 基因的研究进展[J]. 常熟理工学院学报: 自然科学, 2011, 25(8)：76-80.
[20] 李永平, 梁炳生. MyoD 肌形成作用机制研究进展[J]. 国际骨科学杂志, 2007, 28(1)：37-40.
[21] 邱华玲, 于建兴, 陈宏权. MyoG 基因结构与功能研究进展[J]. 生物信息学, 2006, 4: 190-192.