Establishment of Mannose Selection System in Sugarcane Transformation

doi:10.13560/j.cnki.biotech.bull.1985.2015.01.014

Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (1): 92-97.doi: 10.13560/j.cnki.biotech.bull.1985.2015.01.014

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Establishment of Mannose Selection System in Sugarcane Transformation

Wang Wenzhi, Yang Benpeng, Cai Wenwei, Feng Cuilian, Wang Jungang, Xiong Guoru, Zhang Shuzhen

（Sugarcane Research Center of Chinese Academy of Tropical Agricultural Sciences，Institute of Tropical Bioscience and Biotechnology of Chinese Academy of Tropical Agricultural Sciences，Key Laboratory of Biology and Genetic Resources of Tropical Crops Ministry of Agriculture，Haikou 571101）

Received:2014-06-12 Online:2015-01-09 Published:2015-01-10

Abstract

Abstract: In this research the killing curve of mannose was tested at the subculture，differentiation and rooting stages of sugarcane cultivar ROC22. Then the expression cassette containing a pmi selection marker gene and a GFP report gene was transformed into sugarcane embryonic callus by Agrobacterium-mediated method. Resistant plants were obtained after selection by Mannose. The PCR detection result and GFP microscope observation result both show that our lab had established a high efficiency PMI/Mannose selection system in sugarcane transformation.

Key words: sugarcane transgenic, mannose, pmi gene, GFP gene

Wang Wenzhi, Yang Benpeng, Cai Wenwei, Feng Cuilian, Wang Jungang, Xiong Guoru, Zhang Shuzhen. Establishment of Mannose Selection System in Sugarcane Transformation[J]. Biotechnology Bulletin, 2015, 31(1): 92-97.

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Establishment of Mannose Selection System in Sugarcane Transformation

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