Abstract: To identify a strain of actinomycetes JXNU03 through the sequences of 16S rRNA gene. The physiological characteristics were acquired through physiological and biochemical experiments. The antagonistic activity was determined by plate assay and adopting mycelium growth rate method respectively. When the actinomycetes JXNU03 was cultured on Gause 1 medium, the vegetative hyphae were well developed and abundant without fracture, and the aerial hyphae were grown well with straight or spiral spore hypha. There were some thorns on the surface of spores. The glucose was detected in the whole cell hydrolysis, while not other dominant diagnostic sugars, and the L, L-DAP was detected in cell wall. The phylogenetic analysis showed that the level of 16S rRNA gene sequence similarity between the actinomycete JXNU03 and Streptomyes gancidicus NBRC 15412. The strain JXNU03 demonstrated a board spectrum of antagonistic activity against. The Gram-positive bacteria, the Gram-negative bacteria, yeasts and molds. The actinomycetes JXNU03 was identified as Streptomyes gancidicus, and termed Streptomyes gancidicus JXNU03. As its antagonistic activity against bacteria, yeast and filamentous fungi. The actinomycetes JXNU03 possessed an important prospect for antibiotics development.

Key words: Streptomyes gancidicus, phylogenesis, polyphasic taxonomy