Abstract: During the process of Tremella fuciformis cultivation, some problems, such as low yield and long cultivation time, often are followed with different strains. The aim of this study is to mix good properties of different strain together to new strains by sexual hybridization method. Based comparison of yield, fresh ear ventral color and mature culture, yinke 1# and Tr21 were selected as parents strains with good properties. 50 single spores were isolated from fresh ear and partnered each other following single hybridization principle. 17 combinations are identified with an optical microscope examination and 7 genetic characteristics of hybrid strains are different from parent strains by Antagonism experiment. The results of agronomic traits comparison showed that cultivation period, diameter and the yield of hybrid strains GT₃ are significantly different from the parents, and the production has exceeded the parent strains more than 3%. GT₃ can be used as an excellent hybrid strains reserves and has the potential to develop into a new species. Sexual hybridization breeding would be effective way to select good quality strain.

Key words: Tremella fuciformis, single spore isolation, sexual hybridization, good quality strain