Expression of Canine Interferon Alpha in Silkworm-baculovirus Expression System and the Antiviral Activity Assay

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.022

Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (6): 216-220.doi: 10.13560/j.cnki.biotech.bull.1985.2015.06.022

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Expression of Canine Interferon Alpha in Silkworm-baculovirus Expression System and the Antiviral Activity Assay

Li Haoyang¹, Hu Xiaoyuan¹, Yi Yongzhu², Yang Xin¹, Zhang Zhifang¹, Li Yinü¹

1. Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081；2. The Sericultural Research Institute，Chinese Academy of Agricultural Sciences，Zhenjiang 212018

Received:2015-03-17 Online:2015-06-19 Published:2015-06-20

Abstract

Abstract: Canine interferon alpha(CaIFNα)was widely used in the prophylaxis and cure of canine diseases. The purpose of this study is to develop an efficient method to express CaIFNα. A canine interferon alpha gene in GenBank was optimized according to the codon bias of silkworm and synthesized, and then cloned into the baculovirus transfer vector pVL1393 to construct the recombinant plasmid pVL-CaIFNα. The pVL-CaIFNα was co-transfected with the BmBacmid DNA into silkworm cells and after in vivo recombination. The recombinant virus was gathered and used to infect silkworm larvae. The hemolymph of infected larvae was collected for antiviral activity assay. The antiviral activity of expressed CaIFNα was examined on Madin-Darby canine kidney cells infected with the vesicular stomatitis virus expressing green fluorescent protein(VSV*GFP). The result showed that CaIFNα expressed in silkworm can inhibit the multiplication of VSV*GFP and the antiviral activity was about 1.78×10⁶U/mL.

Key words: canine interferon alpha, silkworm-baculovirus expression system, antiviral activity assay

Li Haoyang, Hu Xiaoyuan, Yi Yongzhu, Yang Xin, Zhang Zhifang, Li Yinü. Expression of Canine Interferon Alpha in Silkworm-baculovirus Expression System and the Antiviral Activity Assay[J]. Biotechnology Bulletin, 2015, 31(6): 216-220.

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Expression of Canine Interferon Alpha in Silkworm-baculovirus Expression System and the Antiviral Activity Assay

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