An Analysis of Environmental and Biological Effects of Chlorpyrifos

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.033

Abstract

Abstract: With the prohibition of high-toxicity organophosphorous pesticides, the chlorpyrifos as their substitute has been used in a large-scale. The residue of chlorpyrifos, degrading slowly in the water, may cause the potential damages to aquatic organisms and others. To investigate the endocrine disrupting effects of low-concentration chlorpyrifos, flow cytometry was used to analyze the growth cycle of endometrial cancer cell HEC-1B of human. Moreover, zebrafish embryos were selected for chlorpyrifos exposing treatment 60 h at various concentrations of 0, 1.0, 2.0, 3.0 and 4.0 ppm. It was found that the chlorpyrifos caused the zebrafish embryos to death and significant embryonic malformation, the survival rate of the embryos was inversely proportional to chlorpyrifos’ concentration, and the malformation rate was proportional to chlorpyrifos’ concentration. Further, the mRNA expression levels of 5 neurodevelopment maker genes in the chlorpyrifos-treated zebrafish embryos were detected. These results showed that chlorpyrifos of low-concentration acted as endocrine disruptor, and chlorpyrifos of high-concentration affected the normal development of nerve system.

Key words: chlorpyrifos, environmental and biological effects, zebrafish embryo, developmental neurotoxicity, endocrine disrupting effect

Yu Kaimin, Feng Weimin, Li Guochao, Zhang Jiayu, Liu Lili, Yan Yanchun. An Analysis of Environmental and Biological Effects of Chlorpyrifos[J]. Biotechnology Bulletin, 2015, 31(8): 225-230.

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