Optimization of Suspended Serum-free Medium for BHK-21 Cells by Combining Mixture with Response Surface Analysis

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.009

Abstract

Abstract: This study is to optimize the suspended serum-free medium for BHK-21 cells by combining Mixture experiment design with response surface analysis(RSA). Based on 6 media A-F of known formulation in the laboratory, the optimal combination of the medium with A：B：C：D：E：F = 0：0：11：0：9：0 was screened by the Mixture test. The contents of several key constituents in the medium were optimized by RSA, and the optimal concentrations of glutamine, tyrosine, bovine serum albumin(BSA)and calcium ion were 3 mmol/L, 2.5 g/L, 0 g/L and 0 mmol/L respectively. The serum-free medium effectively supported the suspending growth of BHK cells. The maximum viable cell density of BHK cells in cell suspension culture reached 140.21×10⁵ cells/ml, which was 1.95 times in basal medium. The suspended serum-free medium for BHK-21 cells was developed by combining Mixture experiment design with RSA, which lays a foundation for the industrial production of foot-and-mouth disease(FMD)vaccine while using BHK-21 cells.

Key words: BHK-21 cells, suspended serum-free medium, Mixture experiment design, response surface analysis

Wang Liang, Liu Xuping, Tan Wensong. Optimization of Suspended Serum-free Medium for BHK-21 Cells by Combining Mixture with Response Surface Analysis[J]. Biotechnology Bulletin, 2015, 31(9): 70-78.

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