Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (11): 153-158.doi: 10.13560/j.cnki.biotech.bull.1985.2015.11.020

• Research report • Previous Articles     Next Articles

Cloning and Expression Analysis of the Ethylene-responsive Transcription Factor III-1 Gene cDNA from Melon(Cucumis melo)

Guo Chengyu1,2, Sun Xiaolei1, Shao Qi1, Bai Lihua2, Niu Yiding1, Hasi Agula1   

  1. 1. College of Life Sciences,Inner Mongolia University,Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology,Hohhot 010021;
    2. Bayannaoer Academy of Agricultural and Animal Science,Bayannaoer 015000
  • Received:2015-02-04 Online:2015-11-26 Published:2015-11-26

Abstract: According to the clustering results of the ERF subfamily in melon, CmERFIII-1(accession ID: MELO3C005465)was chosen to be studied. A pair of specific primers was designed based on the cDNA of CmERFIII-1. The full-length cDNA of CmERFIII-1 was cloned by RT-PCR from the fruit of melon(Cucumis melo L.cv. Hetao).The length of cDNA was 711 bp, encoding 236 amino acids. Sequences analysis and the phylogenetic trees showed that the protein amino acid sequence of CmERFIII-1 was closely related to two ERF subfamily member of Cucumis sativus(GeneBank ID: XP_004143677.1、XP_004158119.1). The Real-time quantitative RT-PCR assay showed CmERFIII-1 gene was expressed and its expression levels were different in tissues of the plant and fruits of different developmental stages . The expression level of this gene was highest in leaf.

Key words: melon, ERF, transcription factor, expression analysis

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