The Synergistic Inhibitory Effects of Recombinant Peganum Harmala Lipid Transfer Protein and Cisplatin on the Proliferation of Melanoma B16 Cells in vitro

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.027

Abstract

Abstract: This work is to explore how the combination of recombinant Peganum harmala lipid transfer protein(rPhLTP)and cisplatin(DDP)inhibits the proliferation of melanoma B16 cells and induces the apoptosis of them. MTT assay was used to measure the effects of individual rPhLTP and DDP and combination of them on the proliferation of melanoma B16 cells. The cell apoptosis rate, reactive oxygen species(ROS), and mitochondrial transmembrane potential(Δψm)level were measured using flow cytometry. The results showed that both inhibitory rate and apoptosis rate by the combination of rPhLTP and DDP were significantly higher(P < 0.01)than by individual DDP or rPhLTP, moreover also levels of ROS raised inside cells by the combination were higher than by individuals(P < 0.01). However, the levels of Δψm inside the cells by the combination were lower than those by individuals, but the differences between the combination and individuals were not such significant. In conclusion, rPhLTP enhanced the inhibitory effect of DDP on the proliferation of B16 cells and promoted the cell apoptosis, indicating that rPhLTP and DDP has synergistic antitumor effect on B16 cells.

Key words: recombinant peganum harmala lipid transfer protein, cisplatin, melanoma B16 cells, proliferation inhibition

Chang Chenchen, Wang Yan, Guan Shan, Sun Surong. The Synergistic Inhibitory Effects of Recombinant Peganum Harmala Lipid Transfer Protein and Cisplatin on the Proliferation of Melanoma B16 Cells in vitro[J]. Biotechnology Bulletin, 2015, 31(12): 186-192.

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