Study on the Changes of Soil Microbial Community Structure in Farmland by Estrone Stress

doi:10.13560/j.cnki.biotech.bull.1985.2016-0861

Abstract

Abstract: Estrone is one of natural steroidal estrogens which have female effect and characterizes by high toxicity and refractory. It can accumulate in the body through the food chain,threatening human and animal health. In this study,PCRand DGGE was used to analyze the variation of the microbial community structure in estrone-contaminated farmland soil samples. The results showed that the higher the concentration of estrone,the deeper the effect on the microbial population,and the more complex the phylogenetic relationship among populations. When the concentration of estrone was 500 ng/kg to 2 000 ng/kg,the diversity of microbial community decreased with the increase of E1 concentration;the diversity increased rapidly when the concentration of estrone was over a certain value,and the increase trend of diversity gradually became slower when the concentrations of E1 continuously increased. Estrone in the presence of microorganisms can be transformed to estradiol,estriol and bound-state E1-3G,this transformation brings more complex changes to the microbial community diversity.

Key words: estrone, soil in farmland, microbial community structure, PCR-DGGE

TIAN Lin, ZHANG Xun. Study on the Changes of Soil Microbial Community Structure in Farmland by Estrone Stress[J]. Biotechnology Bulletin, 2017, 33(6): 230-236.

References

[1] Khanal SK, Xie Bin, Thompson ML, et al. Fate, transport and biodegradation of natural estrogens in the environment and engineered systems[J]. Environmental Science and Technology, 2006, 40（21）：6537-6546.
[2] Treviño LS, Wang Q, et al. Hypothesis：Activation of rapid signaling by environmental estrogens and epigenetic reprogramming in breast cancer[J]. Reproductive Toxicology, 2015, 54：136-140.
[3] Tohyama S, Miyagawa S, Lange A, et al. Understanding the molecular basis for differences in responses of fish estrogen receptor subtypes to environmental estrogens[J]. Environmental Science & Technology, 2015, 49（12）：7439.
[4] 曹巧玲, 张俊明, 高志贤, 等. 环境内分泌干扰物研究的进展[J]. 中华预防医学杂志, 2007, 41（3）：224-226.
[5] Kirk L A, Tyler C R, Lye C M, et al. Changes in estrogenic and androgenic activities at different stages of treatment in wastewater treatment works[J]. Environmental Toxicology & Chemistry, 2002, 21（5）：972-979.
[6] Lintelmann J, Katayama A, Kurihara N, et al. Endocrine disruptors in the environment（IUPAC Technical Report）[J]. Pure & Applied Chemistry, 2009, 75（5）：631-681.
[7] 黄斌. 类固醇类内分泌干扰物分析方法及其在滇池水系环境化学行为研究[D]. 昆明：昆明理工大学, 2011.
[8] 胡碧波, 阳春, 张智, 等. 嘉陵江典型城市江段的类固醇雌激素分布特性[J]. 中国给水排水, 2011, 27（21）：54-58.
[9] Vulliet E, Crenolivé C. Screening of pharmaceuticals and hormones at the regional scale, in surface and groundwaters intended to human consumption[J]. Environmental Pollution, 2011, 159（10）：2929-2934.
[10] 李本纲, 崔司宇. 中国天然雌激素排放清单和风险评价[J]. 城市环境与城市生态, 2011（4）：24-28.
[11] Òvreas L, Forney L, Daae FL, Torsvik V. Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA[J]. Applied & Environmental Microbiology, 1997, 63（9）：3367-3373.
[12] Webster G, Embley TM, Prosser JI. Grassland management regimens reduce small-scale heterogeneity and species diversity of beta-proteobacterial ammonia pxidizer populations[J]. Applied & Environmental Microbiology, 2002, 68（1）：20-30.
[13] Cocolin L, Aggio D, Manzano M, et al. An application of PCR-DGGE analysis to profile the yeast populations in raw milk[J]. International Dairy Journal, 2002, 12（5）：407-411.
[14] 刘建林. 雌激素化合物在土壤中的吸附行为及生物降解的研究[D]. 北京：华北电力大学, 2012.
[15] 吴乔龙. 内源甾体雌激素在土壤环境中迁移转化机制研究[D]. 合肥：合肥工业大学, 2014.
[16] 石月娥. 雌激素在畜禽粪便改良农田土壤中的迁移转化及修复[D]. 长春：吉林大学, 2016.
[17] 宋晓明, 杨悦锁, 温玉娟, 等. 雌激素在地下环境中的归宿及其生态调控[J]. 中国环境科学, 2016, （09）：2828-2840.
[18] 张照韩. 城市水体中雌激素的去除机制及复合污染效应研究[D]. 哈尔滨：哈尔滨工业大学, 2010.
[19] 王凌云. 深圳河及沿岸污水处理厂典型雌激素微生物转化研究[D]. 北京：清华大学, 2011.
[20] D’ascenzo G, Di Corcia A, Gentili A, et al. Fate of natural estrogen conjugates in municipal sewage transport and treatment facilities[J]. Science of the Total Environment, 2003, 302（1）：199-209.