Isolation,Identification and Characterization of Arsenic-tolerant Bacteria from Lead-zinc Mine Tailing

doi:10.13560/j.cnki.biotech.bull.1985.2016-0983

Abstract

Abstract: The aim of this study is to isolate highly arsenic-tolerant bacterial strains from heavy metal contaminated soils from the Kangjiawan lead-zinc mine,Hunan Province. Serial dilution and plating method was used to isolate arsenic-tolerant bacteria,and the strains were identified by 16S rRNA sequence analysis. Then the detection of arsenic tolerance-related genes was carried out. The arsenic molybdenum blue experiment was conducted to evaluate arsenic oxidation-reduction ability of arsenic-tolerant bacteria. Furthermore,the indole acetic acid（IAA）produced by the dominant bacterial strains was quantitatively determined. A total of 152 arsenic-tolerant bacterial strains were isolated from soils samples,and six strains were resistant to As⁵⁺ and As³⁺ up to 800 mmol/L and 20 mmol/L,respectively. The six arsenic-tolerant bacterial strains belonged to five different genera,Pseudomonas,Ochrobactrum,Bacillus,Williamsia,and Arthrobacter. In arsenic tolerance-related gene detection experiments,arsenate reductase gene arsCexisted in strains Tw31,Tw133,Sw149,and Tw222,and strains Bw218 and Tw222 contained arsenic ion efflux permease gene arsB/ACR3（2）. Within 72 h,the As³⁺ oxidation rate and As⁵⁺reduction rate of strains Tw133 and Tw222 were higher than those of other strains,which were about 17 % and 35 %,respectively. In particular,strain Tw133 had an As⁵⁺reduction rate of 48.66 % at 144 h. Moreover,the synthetic quantities of IAA in the two strains were 42.86 μg/mL and 24.36 μg/mL,respectively. The isolated strains Tw133 and Tw222 showed obvious advantages in terms of arsenic tolerance,arsenic oxidation-reduction capacity and IAA production,providing experimental materials for the further study of arsenic-tolerant mechanism in bacteria.

Key words: arsenic-tolerant bacteria, arsenic-tolerant gene, arsenic oxidation-reduction, IAA?

WU Dan ZHANG Zhi-peng MA Yu-chao. Isolation,Identification and Characterization of Arsenic-tolerant Bacteria from Lead-zinc Mine Tailing[J]. Biotechnology Bulletin, 2017, 33(5): 210-218.

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