The Affecting Mechanism of 5-aza-2'-deoxycytidine on the NG108-15 Cells in Hypoxia

doi:10.13560/j.cnki.biotech.bull.1985.2016.01.034

Abstract

Abstract: The aim of this study is to explore the mechanisms of 5-aza-2'deoxycytidine(5-Aza-CdR)and/or hypoxia and/or hypoxic preconditioning on the proliferation and cycle of NG108-15 cells while using the inhibitor(5-Aza-CdR)of DNA methyltransferases(DNMTs)to interfere the nerve cell line NG108-15. The 10.0 μmol/L 5-Aza-CdR was added to cell culture medium, and then cells were treated in hypoxia and/or hypoxic preconditioning. The cell morphology was observed under inverted microscope. The proliferation of NG108-15 was measured using the RTCA. The cell apoptosis and cell cycle were analyzed through flow cytometry analysis. The mRNA levels of DNMTs and cell cycle-associated gene were analyzed by real-time PCR. 5-Aza-CdR and hypoxia inhibited NG108-15 cells proliferation, promoted cell early and late apoptosis, and hypoxic preconditioning promoted cell proliferation. However, as cells were treated by hypoxic or hypoxic preconditioning after the cell treated with 5-Aza-CdR, DNMTs and mRNA transcription of cell cycle-associated genes increased. Conclusively, the jointed treatment of 10.0 μM 5-Aza-CdR with hypoxic inhibited the proliferation of nerve cell line NG108-15, and hypoxic preconditioning had a promoting effect on the cell proliferation.

Key words: NG108-15 cell line, 5-aza-2'-deoxycytidine, hypoxic, hypoxic preconditioning

ZHANG Zhu-xia, SUN Ming-ying, YANG Jie, JIANG Shu-yan, YAN Shao-chun, SHAO Guo. The Affecting Mechanism of 5-aza-2'-deoxycytidine on the NG108-15 Cells in Hypoxia[J]. Biotechnology Bulletin, 2016, 32(1): 213-219.

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