Genetic Transformation of Human IL-12 Driven by Tuber-specific Promoter from Potato（Solanum tuberosum）

doi:10.13560/j.cnki.biotech.bull.1985.2016.05.029

Abstract

Abstract: To obtain transgenic potato plants that express human interleukin 12（hIL-12）protein，the early-constructed plant expression vector driven by Ppatatin of potato tuber-specific promotor was transformed into potato by Agrobacterium-mediated transfection，followed by co-cultivation，screening and differentiation，and the transgenic plants were acquired. Techniques of PCR，RT-PCR，GUS staining，and ELISA analysis were performed for the identification of transgenic plants. The results showed that 12 transgenic lines were obtained，and in the 10 transgenic lines the target gene was in the potato genome，i.e.，positive rate was 83%. RT-PCR analysis illustrated that exogenous genes expressed successfully in 7 transgenic lines at mRNA level，moreover，ELISA data showed that 5 transgenic lines presented the high level of hIL-12 protein expression. In addition，the expression of hIL-12 was genetically stable in these 7 transgenic potato lines.

Key words: promoter Ppatatin, human IL-12, transgenic potato, plant bioreactor

YANG Jia-wei,CHENG Xiao-ling,LONG Chao-kang. Genetic Transformation of Human IL-12 Driven by Tuber-specific Promoter from Potato（Solanum tuberosum）[J]. Biotechnology Bulletin, 2016, 32(5): 220-225.

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