A Preliminary Study on Infection Model of Mongolian Gerbil by Hepatitis E Virus

doi:10.13560/j.cnki.biotech.bull.1985.2016.05.032

Abstract

Abstract: To explore the feasibility of using Mongolian gerbils（Meiiones unguiculataus）to construct an animal model of hepatitis E virus（HEV），HEV RNA was extracted from the diluted suspension of swine feces，then Mongolian gerbils were experimentally inoculated with the HEV via intraperitoneal and oral. Subsequently，the samples of blood，relevant organs（heart，liver，spleen，lungs，kidneys，mesenteric lymph nodes，and spleen）and feces were collected weekly. Samples were detected by RT-PCR and immunohistochemistry. As a result，from day 7 after infection，HEV RNA was detected from the blood，kidney，spleen，mesenteric lymph nodes，liver，lung and feces. The analysis of immunohistochemistry discovered that HEV was expressed in the liver，and caused the pathological changes in liver tissue，but it was lack of obvious clinical manifestations in Mongolian gerbils after HEV infection. All above results suggest that Mongolian gerbils have susceptibility to HEV，and have pathological changes of lobular hepatitis in the liver after HEV infection，indicating that Mongolian gerbils have a potential value to be the animal model of HEV infection.

Key words: hepatitis E virus, Mongolian gerbils, animal model

ZHU Guang-ze,QU Yong-gang,LI Yi-quan,LAN Tian,FAN Yuan-yuan,HU Ning-ning,ZHANG Nuo-na,LI Xiao,JIN Ning-yi. A Preliminary Study on Infection Model of Mongolian Gerbil by Hepatitis E Virus[J]. Biotechnology Bulletin, 2016, 32(5): 240-245.

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