Abstract: Conventional methods of studying plant cells rely on growing plant cells in soil pots or agarose plates，followed by screening the plant phenotypes in traditional greenhouses and growth chambers. These methods usually need a large number of experiments，and suffer from low spatial resolution. While the microfluidic chips have many advantages，such as miniaturization，small volume consumption and high-throughput analysis，etc，moreover，it allows to precisely control the micro-environment of plant cells at micron-level. Therefore，it can reduce the cost and experimental time，and achieve in vitro single cell analysis and characterization. In this paper，the materials and manufacturing methods of the microfluidic chip were firstly introduced，then the recent microfluidic chips for studying plant cells were summarized，further mainly the application progress of microfluidic chips in plant root，pollen tube，protoplasts，and cell wall biomechanics were expounded，and finally the future research directions of microfluidic chips for plant cell were prospected.