Cloning and Phylogenetic Analysis of cDNA Sequences Coding for ARC1 and Exo70A1 in Brassica oleraces var. capitata L.

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.012

Abstract

Abstract: Interaction between ARC1 and Exo70A1 in Brassica is a key point of self-incompatibility(SI)signal transduction. In order to study the evolutionary relationship of ARC1 and Exo70A1 from 10 cultivars of SI head cabbages developed in recent years，we successfully cloned and sequenced cDNAs of ARC1 and Exo70A1. Furthermore，by bioinformatics，we had mutation and evolutionary analysis of those coding region sequences，including interaction region and non-interaction regions of ARC1-Exo70A1 from the 10 cabbages and prior published 83 ARC1 and 73 Exo70A1 sequences. The results revealed that：1)The coding nucleic acid sequences of ARC1 and Exo70A1 from the 10 cabbage materials presented parallel evolutionary relationships，and belonged to the Brassica branch. 2)The evolution rate of coding sequence of ARC1 was faster than Exo70A1. 3)The interaction coding region of ARC1-Exo70A1 was obviously in the progress of co-evolution，while the non-interaction coding area of ARC1-Exo70A1 showed significant differentiation rate in evolution. 4)The evolutionary rate of interaction coding area was faster than non-interaction coding region.

Key words: ARC1, Exo70A1, self-incompatibility, evolutionary relationship, co-evolution

YANG Dan, LIAN Xiao-ping, ZHOU Yan, ZHANG He-cui, DU Dan, GAO Qi-guo, REN Xue-song, ZHU Li-quan. Cloning and Phylogenetic Analysis of cDNA Sequences Coding for ARC1 and Exo70A1 in Brassica oleraces var. capitata L.[J]. Biotechnology Bulletin, 2016, 32(6): 76-88.

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