Biotechnology Bulletin ›› 2016, Vol. 32 ›› Issue (6): 238-243.doi: 10.13560/j.cnki.biotech.bull.1985.2016.06.035

• Research report • Previous Articles     Next Articles

Lipid Production from the Photoautotrophy of Three Strains of Scenedesmus Species

CHEN Chuan-hong, WU Hong, LI Qing, YIN Shun-ji, LUO Shao-jing, CUI Chun-li   

  1. State Key Laboratory of Coal-based Low-carbon Energy,Xin’ao Scientific & Technological Developmental Co.,Ltd.,Langfang 065001
  • Received:2015-09-30 Online:2016-06-27 Published:2016-06-28

Abstract: The growth and the lipid content of 3 strains of Scenedesmus were compared by column photobioreactor,and it was concluded that the strain of producing the highest biomass was Scenedesmus deserticola with 0.48 g/(L·d),followed by Scenedesmus sp(S. sp)0.41 g/(L·d),and the lowest by S. dimorphus with 0.35 g/(L·d). In the late culture stage,the lipid distribution was clearly visible by Nile red staining. The total lipid content and the lipid productivity of S. deserticola were 55.3 % of DCW and 0.29 g/(L·d),respectively,followed by S. dimorphus in 46.7 % and 0.18 g/(L·d),S. sp in 43.6 % and 0.20 g/(L·d). The main fatty acid composition of 3 Scenedesmus were C18 and C16,accounting for over 85% of total fatty acids,satisfying the requirements of biodiesel production. Comprehensive comparison revealed that the S. deserticola was a lipid-producing strain with high performance.

Key words: Scenedesmus species, growth, Nile red, biomass, lipid