Identification and Biological Efficacy of Antagonistic Strain Lh-1 Suppress Watermelon Fusarium Wilt

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.016

Abstract

Abstract: Aims：Identify the strain Lh-1 and evaluate its capability to suppress watermelon Fusarium wilt. Methods：Test the morphological,physiological and 16S rRNA gene sequence characteristics of strain Lh-1. Detect its antagonistic spectrum. A pot experiment was designed to evaluate the effects of applying strain Lh-1 combined with organic fertilizer（T2）,compared with an organic fertilizer（T1）and a chemical fertilizer（CK）. Results：the strain Lh-1 was identified as Bacillus amyloliquefaciens. It can suppress a variety of plant pathogens with a broad-spectrum antimicrobial activity,especially for F. oxysporum f. sp. niveum. Pot experiment showed that the disease incidence is only 17.2%,the control efficacy reached 78.5%,the SOD,POD and CAT activity increased significantly and the MDA content reduced obviously. The number of bacteria and actinomycetes increased but the number of fungi,especially Fusarium oxysporum decreased obviously in the rhizosphere microflora after Lh-1 treated. Conclusion：the application of theBacillus amyloliquefaciens Lh-1 can suppressed watermelon Fusarium wilt.

Key words: B. Amyloliquefaciens, watermelon Fusarium wilt, F. Oxysporum, rhizosphere microflora

ZHAO Jia, HUANG Jing, CHEN Zhe, NIE Yuan-jun, LIANG Hong. Identification and Biological Efficacy of Antagonistic Strain Lh-1 Suppress Watermelon Fusarium Wilt[J]. Biotechnology Bulletin, 2017, 33(4): 130-136.

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