An Evaluation on Heat Tolerance of a Desert Plant Karelinia caspia Seedlings

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.020

Abstract

Abstract: In order to explore and utilize the feature of heat tolerance of a desert plant Karelinia caspia,its heat tolerance during K. caspia seedling was evaluated. The 5 physiological indexes,relative conductivity,MDA content,activity variation of SOD,POD and CAT,as well as trypan blue staining in the K. caspia seedling leaves were measured at varied timeslots under different temperature treatments（40,45 and 50℃）by traditional methods. The reliability of using these indexes in the evaluation of the heat tolerance of K. caspia was comprehensively analyzed by subordinate function method. Results showed that：（1）Under 40℃,the relative conductivity and MDA content tended to increase during early stage of treatment and then reduce in K. caspia seedling with the stress time increasing. The activities of SOD,POD and CAT rose with treatment time longing. There was no coloration on the leaves of K. caspia by trypan blue staining.（2）Under 45℃,each index remained stable at 2-8 h after treatment. The MDA content and relative conductivity rose sharply at 12 h after treatment,but the enzymatic activities declined. With the extension treatment time,the relative coloration area of K. caspia leaves by trypan blue staining enlarged gradually.（3）Under 50℃,the MDA content and relative conductivity rose gradually to the significant level. The activities of SOD,POD,and CAT reached peak at 1 h after treatment,but rapidly decreased as treating time extending. With the extension of treatment time,the relative coloration area of K. caspia leaves by trypan blue staining enlarged gradually,and the color became heavily. The results of subordinate function method showed that these 5 physiological indexes could be used as the indicators of evaluating the feature of heat tolerance in K. caspia. In addition,theK. caspia was not susceptible to 40℃,and the tolerant time was 8 h under 45℃ and 1 h under 50℃,indicating that K. caspia is a highly tolerant plant to high temperature.

Key words: Karelinia caspia L., high temperature stress, tolerance to heat stress

WANG Yan-qin, SHI Xin-jian, LI Zhi-jun. An Evaluation on Heat Tolerance of a Desert Plant Karelinia caspia Seedlings[J]. Biotechnology Bulletin, 2017, 33(4): 157-163.

References

[1] Krasensky J, Jonak C. Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks[J]. J Exp Bot, 2012, 63（4）：1593-1608.
[2] Mittler R, Finka A, Goloubinoff P. How do plants feel the heat?[J]. Trends in Biochem Sci, 2012, 37（3）：118-125.
[3] 罗家雄, 程昌平, 丁金石, 等. 花花柴利用的研究[J]. 中国草原, 1988, 2：16-20.
[4] 周玲玲, 冯元忠, 吴玲, 等. 新疆六种盐生植物的解剖学研究[J]. 石河子大学学报：自然科学版, 2002, 3：217-221.
[5] 王翠, 雷加强, 李生宇, 等. 新疆策勒绿洲-沙漠过渡带花花柴沙堆的形态特征[J]. 中国沙漠, 2013, 33（4）：981-989.
[6] 唐钢梁, 李向义, 林丽莎, 等. 表皮环割对花花柴水势及光合参数的短期影响[J]. 中国沙漠, 2014, 34（6）：1527-1536.
[7] 李合生. 植物生理生化实验原理和技术[M]. 北京：高等教育出版社, 2000：105-118.
[8] 陈建勋, 王晓峰. 植物生理学实验指导[M]. 第2版：广州：华南理工大学出版社, 2006：64-66.
[9] 陈德明, 俞仁培, 杨劲松. 盐渍条件下小麦抗盐性的隶属函数值法评价[J]. 土壤学报, 2002, 39（3）：368-374.
[10] Pucciariello C, Banti V, Perata P. ROS signaling as common element in low oxygen and heat stresses[J]. Plant Physiol and Biochem, 2012, 59：3-10.
[11] Jaspers P, Kangasjärvi J. Reactive oxygen species in abiotic stress signaling[J]. Physiol Plantarum, 2010, 138（4）：405-413.
[12] 张志伟, 周津吟, 李莎. 高温胁迫对胭脂花叶片细胞膜透性影响研究[J]. 安徽农学通报, 2015, 21（3-4）：33-34.
[13] Saidi Y, Finka A, Goloubinoff P. Heat perception and signalling in plants：a tortuous path to thermotolerance[J]. New Phytologist, 2011, 190：556-565.
[14] Wang SY, Gao HY. Effect of chitosan -based edible coating on antioxidants, antioxidant enzyme system, and postharvest fruit quality of strawberries（Fragaria×aranassa Duch. ）[J]. Food Science and Technology, 2013, 52（2）：71.
[15] 杨小飞, 郭房庆. 高温逆境下植物叶片衰老机理研究进展[J]. 植物生理学报, 2014, 50（9）：1285-1292.
[16] 赵森, 于江辉, 肖国樱. 高温胁迫对爪哇稻剑叶抗氧化酶及膜透性的影响[J]. 热带作物学报, 2012, 33（10）：1846-1850.
[17] 朱鑫, 沈火林. 高温胁迫对芹菜幼苗细胞膜稳定性的影响[J]. 北方园艺, 2014（7）：16-20.
[18] 屠小菊, 汪启明, 饶力群. 高温胁迫对植物生理生化的影响[J]. 湖南农业科学, 2013（13）：28-30.
[19] 吴韶辉, 朱美红. 柑橘高温热害生理作用机理研究进展[J]. 浙江柑橘, 2013, 30（2）：7-11.
[20] 王丽娟, 欧静, 钟登慧, 等. 高温胁迫对桃叶杜鹃幼苗生理生化指标的响应[J]. 湖北农业科学, 2014, 20：4882-4891.
[21] 耿兴敏, 胡才民, 杨秋玉, 等. 杜鹃花对各种非生物逆境胁迫的抗性研究进展[J]. 中国野生植物资源, 2014, 3：18-21.