Cloning and Activity Analysis of Promoter of GRAS Transcription Factor Gene HcSCL13 from Halostachys caspica

doi:10.13560/j.cnki.biotech.bull.1985.2017.05.019

Abstract

Abstract: In order to explore the expression characteristic and function of GRAS transcription factor gene HcSCL13 from Halostachys caspica,the 2 200 bp sequence of promoter was cloned using the genomic walking method. The analysis results using the PlantCARE software suggested that the promoter sequence contained not only the core elements such as CAAT-box and TATA-box,but also some cis-element related to the stress response. The 35S promoter sequence of the pBI121 expression vector was replaced by the HcSCL13promoter sequence to construct the fusion expression vector. Then,the fusion expression vector was transformed into Arabidopsis thaliana by flora method and the T1 seedlings were stained by GUS. The results of GUS staining showed that the whole transformed A. thalianaseedlings were stained,indicating that the HcSCL13 gene promoter had expression activity,and it might be constitutive promoter.

Key words: transcription factor gene HcSCL13, genomic walking method, promoter cloning, analysis of cis-acting element and promoter activity

FAN Shou-de WANG Yan. Cloning and Activity Analysis of Promoter of GRAS Transcription Factor Gene HcSCL13 from Halostachys caspica[J]. Biotechnology Bulletin, 2017, 33(5): 131-138.

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