Isolation of Rare Actinobacteria in 5 Ecodistricts of Tarim Basin and Distribution of the Genes Synthesizing Antibiotics

doi:10.13560/j.cnki.biotech.bull.1985.2018-0011

Abstract

Abstract: This work is for investigating the distribution of rare actinobacteria in different ecodistricts and assessing the potential of antibiotic production of these actinobacteria. Total 5 soil samples were collected from the Tarim Basin,and rare actinobacteria were isolated from these samples by 7 media. And then the potential of antibiotic productions was assessed by detecting type I PKS,type II PKS,NRPS,APH and HMG-CoA genes,related to antibiotics synthesis. The results showed that：（1）by molecular identification,we obtained 18 kinds of rare actinobacteria strains belonging to 10 different genera;（2）the riparian forest ecodistrict in the Tarim Basin had the most species of rare actinobacteria,and there were 8 genera;while the least one was the east of the Talimu river ecodistrict in Taklimakan desert,and there were 4 genera;（3）9 of these 18 strains had type I PKS gene,4 strains had type II PKS gene and APH gene,and 3 strains had NRPS gene;there was one Streptosporangium strain that contained type I PKS gene,type II PKS gene,NRPS gene and APH gene. Conclusively,5 ecological zones are found to have relatively more rare actinomycetes which also contain abundant genes in associated with the synthesis of antibiotics.

Key words: Tarim Basin, ecodistrict, rare actinobacteria, functional genes screening

ZHU Rong-gui, GUAN Tong-wei, JIANG Xiu-juan. Isolation of Rare Actinobacteria in 5 Ecodistricts of Tarim Basin and Distribution of the Genes Synthesizing Antibiotics[J]. Biotechnology Bulletin, 2018, 34(9): 230-236.

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