Analysis of miRNA Transcriptome in Early Developmental Stage and Identification of Growth-related miRNA of Siniperca chuatsi

doi:10.13560/j.cnki.biotech.bull.1985.2018-0261

Abstract

Abstract: MicroRNA（miRNA）is a kind of non-coding small RNA molecules with the length of about 20-22 nt,and they are involved in regulating the expression of target genes at the post-transcriptional level. High-throughput sequencing technology was employed to analyze the miRNA transcriptome of the whole larvae of Siniperca chuatsi at day 3,day 17 and day 28 after hatching. A total of 1084 miRNAs were identified,including 432 known,624 conserved,and 28 new miRNAs. miRNAs of the 17-day larvae were identified the most with 926. The 628 miRNAs were ubiquitously expressed at all the days sampled,while 71,104 and 70 miRNAs were specially expressed at day 3,day 17 and day 28,respectively. Several miRNAs that were involved in development showed sustained up or down regulation patterns. Further results suggested that miR-199-5p/miR-203 were correlated with the growth and development of mandarin fish,and the corresponding target genes were WnT and MyoD,respectively.

Key words: mandarin fish, high-throughput sequencing, microRNA, development, growth

ZHAO Yan, CAO Xiao-ying, ZHOU Hao-tian, SONG Ling-yuan, TU Han-qing, HUANG Si-ying, ZHAO Jin-liang. Analysis of miRNA Transcriptome in Early Developmental Stage and Identification of Growth-related miRNA of Siniperca chuatsi[J]. Biotechnology Bulletin, 2018, 34(8): 181-189.

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