Functional Analysis of Arginine N-methyltransferase Gene MoHMT1 in Magnaporthe oryzae

doi:10.13560/j.cnki.biotech.bull.1985.2018-0313

Abstract

Abstract: Arginine methylation regulated by protein arginine methyltransferases（PRMTs）is a common post-translational modification in eukaryotic organisms. In order to analyze the functions of gene MoHMT1（hnRNP arginine N-methyltransferase,PRMT1 homologous）,Mohmt1-knockout mutants were obtained based on the principle of homologous recombination,and preliminarily phenotypic characterizations were carried out. The results showed that the deletion of Mohmt1 resulted in the smaller colony,thinner aerial hyphae,and significantly slower growth of Magnaporthe oryzae,compared to the wild type. The conidia amount produced by Mohmt1-deleted mutants demonstrated obviously reduction with only 20% of the wild type;however,the conidia produced by the mutants normally germinated to form appressorium and successfully penetrated the onion epidermal cells. Furthermore,using the conidia suspension to inoculate the tested rice Leaves,it was found that the number of Lesions produced by the MoHmt1-deleted mutants significantly decreased related to the wild type and deletion of MoHmt1 attenuated pathogenicity on susceptible rice. These results indicate that MoHMT1 protein may play important regulation role in the hyphal development and pathogenic process of M. oryzae.

Key words: Magnaporthe oryzae, MoHMT1, gene knockout, growth and development, pathogenicity

ZHANG Wen-ze, ZHANG Yan-Li, MEN Yan-ming, ZHANG Yu-jiao, SUN Zhi-xin, LI Wen-hui, LU Guo-dong, QI Yao-yao. Functional Analysis of Arginine N-methyltransferase Gene MoHMT1 in Magnaporthe oryzae[J]. Biotechnology Bulletin, 2019, 35(12): 38-44.

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