Abstract: In order to obtain a large number of embryogenic calluses of Pinus elliottii being resistant to brown spot needle blight of pine and to improve the technique of somatic embryogenesis from the resistant embryogenic callus,the suitable conditions of somatic cell suspension culture of P. elliottii were studied,and the suspension culture system of somatic cells of P. elliottii resistant to brown spot needle blight of pine was established. The results showed that suspension culture of embryogenic cells was more dispersed,which was conducive for embryonic cells to have full nutrition,and 153 mature somatic embryos per gram was produced,the embryogenic cells produced in solid culture per gram were only 56. The optimal culture period of suspension cells of resistant P. elliottii was 7-9 d,the suitable basic medium was 1/4 DCR,the dosage of maltose was 15 g/L,NAA was 0.5 mg/L,and the optimal subculture addition ratio was 1/2. Meanwhile under the same culture conditions,the viabilities of suspension cells of resistant P. elliottii in different genotypes varied,and that of clone 13#-9 was the best in this experiment.

Key words: resistant Pinus elliottii, suspension culture, somatic embryogenesis, embryogenic callus