Using Escherichia coli Light-regulated Gene Expression System to Degrade MBC

doi:10.13560/j.cnki.biotech.bull.1985.2018-0742

Abstract

Abstract: A newly developed Escherichia coli light-regulated gene expression system was used to produce methyl-1h-benzimidazol-2-yl carbamate（MBC）hydrolyzing esterase for degrading residual pesticide MBC in environment. This light-regulated system is based on dimerization of photosensitive protein LEV1 upon light exposure to repress the expression of downstream gene. SDS-PAGE and ELISA results showed that the gene of MBC hydrolyzing esterase could be overexpressed in our light-regulated system,by which the MBC hydrolyzing esterase was produced and it had enzymatic activity of degrading pesticide MBC in both liquid solution and soil samples. Light as an inducer,given its many advantages such as cheap,easy to be obtained,non-toxic,and added/removed at any time,is a promising tool for the industrial production of enzymes.

Key words: Escherichia coli, light-regulated gene expression system, photosensitive protein, MBC hydrolyzing esterase, degradation

YU Shu-qiao, GUAN Zhao-ying, CHEN Hong. Using Escherichia coli Light-regulated Gene Expression System to Degrade MBC[J]. Biotechnology Bulletin, 2019, 35(2): 218-224.

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