Isolation of Cold-adapted Cellulose-degrading Bacteria Using Three Different Carbon Sources and Analysis on the Degrading Ability of Consortia

doi:10.13560/j.cnki.biotech.bull.1985.2018-0956

Abstract

Abstract: The cold-adapted cellulose-degrading bacteria were screened from the forest soil of Hulun Buir city using avicel,carboxymethylcellulose（CMC）and D-salicin as carbon source respectively at 4℃. The strains of high enzyme activity were respectively mixed to construct cold-adapted cellulose-degrading consortia and their filter paper degradation abilities were measured. As a result,172 strains of cold-adapted cellulose-degrading bacteria were isolated. Of them,the bacteria isolated with avicel or CMC as carbon source belonged to 4 classes,and the bacteria isolated with D-salicin as carbon source belonged to 6 classes. The first dominant class was γ-Proteobacteria with ratio of 54%,48% and 55%,respectively;the second dominant class was α-Proteobacteria with ratio of 28%,26% and 34% respectively. The first dominant genus was Pseudomonaswith ratio of 35%,26% and 26% respectively,the second dominant genus screened with avicel as carbon source was Rhizobium with ratio of 15%,the second dominant genera screened with CMC as carbon source were Rhizobium and Oerskasty with ratio of 12%,the second dominant genus screened with D-salicin as carbon source was Lelliottia with ratio of 19%. In 27 cold-adapted cellulose-degrading consortia,D13'1'' and D13'2'' presented the highest filter paper enzyme activity of 158.02 U/mL,which was 5-10 times more than that of any single strain in its consortia. D13'1'' and D13'2'' are the optimal flora combination of cold-adapted cellulose-degrading consortia with high application potential.

Key words: cold-adapted, cellulose-degrading bacteria, consortia, filter paper enzyme activity, carbon source

MENG Jian-yu, JI Jin-hua, JIA Li-juan, GUO Hui-qin, TAO Yu, FENG Fu-ying. Isolation of Cold-adapted Cellulose-degrading Bacteria Using Three Different Carbon Sources and Analysis on the Degrading Ability of Consortia[J]. Biotechnology Bulletin, 2019, 35(8): 77-84.

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