Effects of Small Molecule Precursors on Bafilomycin A1 Biosynthesis

doi:10.13560/j.cnki.biotech.bull.1985.2018-1001

Abstract

Abstract: The objective of this work is to explore an effective method of increasing bafilomycin A1 productivity via understanding the effects of small molecule precursors on bafilomycin A1 biosynthesis. The effects of different precursors on the biosynthesis of bafilomycin A1,the optimal concentration and feeding time of the effective precursor were mainly studied. As result,valine was the optimal precursor for bafilomycin A1 biosynthesis. By feeding of 0.2% valine into the fermentation broth at the 36th hour of cultivation,the bafilomycin A1 production reached 285.5 mg/L,which was 78.4% higher than that in the condition without any addition of precursor. In conclusion,feeding precursors significantly influences bafilomycin A1 biosynthesis,and the addition of valine is effective in increasing bafilomycin A1 production.

Key words: bafilomycin A1, biosynthesis, fermentation, precursor

ZHOU Jian, SUN Fei, FANG Zhi-kai, JIANG Hong. Effects of Small Molecule Precursors on Bafilomycin A1 Biosynthesis[J]. Biotechnology Bulletin, 2019, 35(6): 125-130.

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