Cloning and Expression Analysis of SikCML7 Gene from Saussurea involucrata

doi:10.13560/j.cnki.biotech.bull.1985.2018-1056

Abstract

Abstract: Calmodulin-like protein is one of the most important Ca²⁺ receptors and participates in the adaptation process of plants to environment. In order to explore whether or not calmodulin-like proteins are involved in the plant response to stress,the SikCML7 gene was cloned based on the low temperature transcriptome of Saussurea involucrata. Bioinformatics analysis indicated that the open reading frame of SikCML7 was 450 bp and encoded 149 amino acids,and there were 4 calcium binding regions（EF-hands）in the encoded proteins. Phylogenetic tree analysis indicated that this gene was close to Arabidopsis AtCML9 in the evolutionary affinity. The results of real-time quantitative PCR showed that the expression of SikCML7gene was up-regulated after treatment with two different low temperatures（4℃/-2℃）,drought and salt stress respectively;however this gene demonstrated significantly different expression patterns under chilling stress and freezing stress as well as the certain responses to drought and salt stress. In sum,SikCML7 indeed participates in the responses process of S. involucrata to low temperature,draught,and salt stresses.

Key words: Saussurea involucrate, calmodulin-like protein, adverse stress, expression analysis

LIU Zhao-shu, LIU Yuan-yuan, QIN Yu-jie, BI Quan, WANG Sai-sai, ZHU Jian-bo. Cloning and Expression Analysis of SikCML7 Gene from Saussurea involucrata[J]. Biotechnology Bulletin, 2019, 35(6): 48-54.

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