Establishment of NF-κB Luciferase Reporter Cell Line with Commonality

doi:10.13560/j.cnki.biotech.bull.1985.2019-0058

Abstract

Abstract: This work is aimed to establish luciferase reporter gene system with commonality. Lentivirus containing NF-κB-RE-Luc2P was transduced into HEK293 and monoclonal HEK293 cell line expressing luciferase reporter gene under control of NF-κB response element was established and verified with TNF-α stimulation. To demonstrate the cell line capacity of commonality and modification,FGF family receptor FGFRⅡwas further transduced into this cell line and the screened FGFRⅡ/NF-κB-RE-Luc2P HEK293 single clones was further validated by FGF analogue stimulation. Dose-response effect and more than 100 fold of signal to noise ratio were observed in this cell line in TNF-α stimulation,indicating the cell line was generated successfully. In response to FGF analogue stimulation,dose-response effect was fitted as the “S” curve,implying that the cell line can be applied to the activity detection of FGF-type medicine. The results showed that NF-κB-RE-Luc2P HEK293 and modified one FGFRⅡ/NF-κB-RE-Luc2P HEK293 was established. that the cell line has commonality regarding NF-κB signaling.

Key words: NF-κB;, FGFRⅡ, commonality, luciferase reporter gene

ZHENG Hai-liang, WANG Dong-fang, FANG Ya, DIAO Xiao-wei, WEN Yong, YUAN Yun-xia. Establishment of NF-κB Luciferase Reporter Cell Line with Commonality[J]. Biotechnology Bulletin, 2019, 35(7): 129-133.

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