Research of Progress of Strategy and Application of Metabolic Pathway Modification in Escherichia coli

doi:10.13560/j.cnki.biotech.bull.1985.2019-0131

Abstract

Abstract: The metabolic pathway of Escherichia coli can be modified by genetic engineering,which can be used for the synthesis of biofuels,chiral drugs,and their derivatives. E. coli has become a common host microorganism for genetic modification due to its strong stability and easy operation. Using metabolomics and synthetic biology,E. coli can be efficiently used as a target for biocatalytic production. This paper reviews the strategies of metabolic pathway modification of pyruvate,acetyl coenzyme A,mevalonate and shikimic acid in E. coli,and the application of metabolic pathway modification of E. coli in the synthesis of biofuels and block compounds,which provides a holistic idea and method for researchers to study the synthesis of target compounds by E. coli.

Key words: Escherichia coli, metabolic pathway, biofuel, block compounds

LI Ran, HUANG Yu-qing, JIA Zhen-hua. Research of Progress of Strategy and Application of Metabolic Pathway Modification in Escherichia coli[J]. Biotechnology Bulletin, 2019, 35(8): 232-237.

References

[1] Li Y, Chen J, Lun SY.Biotechnological production of pyruvic acid[J]. Applied Microbiology & Biotechnology, 2001, 57(4):451-459.
[2] Atsumi S, Hanai T, Liao JC.Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels[J]. Nature, 2008, 451(7174):86-89.
[3] Nielsen DR, Yoon SH, Yuan CJ, et al.Engineering Acetoin and meso-2, 3-butanediol biosynthesis in E. coli[J]. Biotechnology Journal, 2010, 5(3):274-284.
[4] 宋灿辉, 张伟国. 敲除aceE基因对大肠杆菌生长和丙酮酸代谢的影响[J]. 生物加工过程, 2013, 11(6):15-18.
[5] Causey TB, Zhou S, Shanmugam KT, et al.Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products:Homoacetate production[J]. Proceedings of the National Academy of Sciences, 2003, 100(3):825-832.
[6] Causey TB, Shanmugam KT, Yomano LP, et al.Engineering Escherichia coli for efficient conversion of glucose to pyruvate[J]. Proceedings of the National Academy of Sciences, 2004, 101(8):2235-2240.
[7] Zelić B, Gostović S, Vuorilehto K, et al.Process strategies to enhance pyruvate production with recombinant Escherichia coli:From repetitive fed-batch to in situ product recovery with fully integrated electrodialysis[J]. Biotechnology & Bioengineering, 2010, 85(6):638-646.
[8] Zhu Y, Eiteman MA, Dewitt K, et al.Homolactate fermentation by metabolically engineered Escherichia coli strains[J]. Applied & Environmental Microbiology, 2007, 73(2):456-464.
[9] Atsumi S, Cann AF, Connor MR, et al.Metabolic engineering of Escherichia coli for 1-butanol production[J]. Metabolic Engineering, 2007, 10(6):305-311.
[10] Shen CR, Lan EI, Dekishima Y, et al.Driving forces enable high-titer anaerobic 1-butanol synthesis in Escherichia coli[J]. Applied & Environmental Microbiology, 2011, 77(9):2905-2915.
[11] Baek JM, Mazumdar S, Lee SW, et al.Butyrate production in engineered Escherichia coli with synthetic scaffolds[J]. Biotechnology and Bioengineering, 2013, 110(10):2790-2794.
[12] Gulevich AY, Skorokhodova AY, Sukhozhenko AV, et al.Biosynthesis of enantiopure(S)-3-hydroxybutyrate from glucose through the inverted fatty acid β-oxidation pathway by metabolically engineered Escherichia coli[J]. Journal of Biotechnology, 2017, 244:16-24.
[13] Jian J, Zhang SQ, Shi ZY, et al.Production of polyhydroxyalkanoates by Escherichia coli mutants with defected mixed acid fermentation pathways[J]. Applied Microbiology & Biotechnology, 2010, 87(6):2247-2256.
[14] Kim Y, Ingram LO, Shanmugam KT.Dihydrolipoamide dehydrogenase mutation alters the NADH sensitivity of pyruvate dehydrogenase complex of Escherichia coli K-12[J]. Journal of Bacteriology, 2008, 190(11):3851.
[15] Boldt J, Khandurina J, Trawick JD, et al.Metabolic engineering of Escherichia coli for direct production of 1, 4-butanediol[J]. Nature Chemical Biology, 2011, 7(7):445-452.
[16] Bond-Watts BB, Bellerose RJ, Chang MCY.Enzyme mechanism as a kinetic control element for designing synthetic biofuel pathways[J]. Nature Chemical Biology, 2011, 7(4):222-227.
[17] Xu P, Gu Q, Wang W, et al.Modular optimization of multi-gene pathways for fatty acids production in E. coli[J]. Nature Communications, 2013, 4:1409.
[18] Lin H, Castro NM, Bennett GN, et al.Acetyl-CoA synthetase overexpression in Escherichia coli demonstrates more efficient acetate assimilation and lower acetate accumulation:a potential tool in metabolic engineering[J]. Applied Microbiology & Biotechnology, 2006, 71(6):870-874.
[19] Martin VJJ, Pitera DJ, Withers ST, et al.Engineering a mevalonate pathway in Escherichia coli for production of terpenoids[J]. Nature Biotechnology, 2003, 21(7):796-802.
[20] Harada H, Yu F, Okamoto S, et al.Efficient synthesis of functional isoprenoids from acetoacetate through metabolic pathway-engineered Escherichia coli[J]. Applied Microbiology and Biotechnology, 2008, 81(5):915-925.
[21] Morrone D, Lowry L, Determan MK, et al.Increasing diterpene yield with a modular metabolic engineering system in E. coli:comparison of MEV and MEP isoprenoid precursor pathway engineering[J]. Applied Microbiology and Biotechnology, 2010, 85(6):1893-1906.
[22] Krämer M, Bongaerts J, Bovenberg R, et al.Metabolic engineering for microbial production of shikimic acid[J]. Metabolic Engineering, 2003, 5(4):277-283.
[23] Dewick PM.The biosynthesis of shikimate metabolites[J]. Natural Product Reports, 1990, 7(3):165-189.
[24] Draths KM, David RK, Frost JW.Shikimic acid and quinic Acid:Replacing isolation from plant sources with recombinant microbial biocatalysis[J]. Journal of the American Chemical Society, 1999, 121(7):1603-1604.
[25] Noda S, Shirai T, Oyama S, et al.Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives[J]. Metabolic Engineering, 2016, 33:119-129.
[26] Gosset G, Yongxiao J, Berry A.A direct comparison of approaches for increasing carbon flow to aromatic biosynthesis in Escherichia coli[J]. Journal of Industrial Microbiology, 1996, 17(1):47-52.
[27] Sengupta S, Jonnalagadda S, Goonewardena L, et al.Metabolic engineering of a novel muconic acid biosynthesis pathway via 4-hydroxybenzoic acid in Escherichia coli[J]. Applied and Environmental Microbiology, 2015, 81(23):8037-8043.
[28] Shen CR, Liao JC.Metabolic engineering of Escherichia coli for 1-butanol and 1-propanol production via the keto-acid pathways[J]. Metabolic Engineering, 2008, 10(6):312-320.
[29] Choi Y, Choi YJ, Park J, et al.Metabolic engineering of Escherichia coli for the production of 1-propanol[J]. PLoS One, 2012, 14(5):477-486.
[30] Jain R, Yan Y.Dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli[J]. Microbial Cell Factories, 2011, 10(1):1-10.
[31] Hou X, Peng W, Xiong L, et al.Engineering Clostridium acetobutylicum for alcohol production[J]. Journal of Biotechnology, 2013, 166(1-2):25-33.
[32] Jang YS, Lee JY, Lee J, et al.Enhanced butanol production obtained by reinforcing the direct butanol-forming route in clostridium acetobutylicum[J]. MBio, 2012, 3(5):e00314-12.
[33] Lee JY, Yu SJ, Lee J, et al.Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production[J]. Biotechnology Journal, 2010, 4(10):1432-1440.
[34] Shen CR, Lan EI, Dekishima Y, et al.Driving forces enable high-titer anaerobic 1-butanol synthesis in Escherichia coli[J]. App-lied & Environmental Microbiology, 2011, 77(9):2905-2915.
[35] Choi YJ, Lee SY.Microbial production of short-chain alkanes[J]. Nature, 2013, 502(7472):571-574.
[36] Schirmer A, Rude MA, Li X, et al.Microbial biosynthesis of alkanes[J]. Science, 2010, 329(5991):559-562.
[37] Henry L, Bennett GN, San KY.Fed-batch culture of a metabolically engineered Escherichia coli strain designed for high-level succinate production and yield under aerobic conditions[J]. Biotechnology & Bioengineering, 2010, 90(6):775-779.
[38] Jantama K, Haupt MJ, Svoronos SA, et al.Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate[J]. Biotechnology & Bioengineering, 2010, 99(5):1140-1153.
[39] Sanchez AM, Bennett GN, San KY.Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity[J]. Metabolic Engineering, 2005, 7(3):229-239.
[40] Yang J, Wang Z, Zhu N, et al.Metabolic engineering of Escherichia coli and in silico comparing of carboxylation pathways for high succinate productivity under aerobic conditions[J]. Microbiological Research, 2014, 169(5-6):432-440.
[41] Boldt J, Khandurina J, Trawick JD, et al.Metabolic engineering of Escherichia coli for direct production of 1, 4-butanediol[J]. Nature Chemical Biology, 2011, 7(7):445-452.
[42] Fong SS, Burgard AP, Herring CD, et al.In silico design and adaptive evolution of Escherichia coli for production of lactic acid[J]. Biotechnology & Bioengineering, 2010, 91(5):643-648.
[43] Jiang XL, Meng X, Xian M.Biosynthetic pathways for 3-hydroxypropionic acid production[J]. Applied Microbiology and Biotechnology, 2009, 82(6):995-1003.
[44] Tseng HC, Harwell CL, Martin CH, et al.Biosynthesis of chiral 3-hydroxyvalerate from single propionate-unrelated carbon sources in metabolically engineered E. coli[J]. Microbial Cell Factories, 2010, 9(1):96.
[45] Tseng HC, Martin CH, Nielsen DR, et al.Metabolic engineering of Escherichia coli for enhanced production of(R)- and(S)-3-hydroxybutyrate[J]. Applied and Environmental Microbiology, 2009, 75(10):3137-3145.
[46] Kim HJ, Hou BK, Lee SG, et al.Genome-wide analysis of redox reactions reveals metabolic engineering targets for d-lactate overproduction in Escherichia coli[J]. Metabolic Engineering, 2013, 18:44-52.
[47] Mazumdar S, Blankschien MD, Clomburg JM, et al.Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coli[J]. Microbial Cell Factories, 2013, 12:7.
[48] Varman AM, Yu Y, You L, et al.Photoautotrophic production of D-lactic acid in an engineered cyanobacterium[J]. Microbial Cell Factories, 2013, 12(1):117.