Research on SNP Associated with Multiparous Trait in Double-lamb of Tan Sheep by SNaPshot Assay

doi:10.13560/j.cnki.biotech.bull.1985.2019-0167

Abstract

Abstract: The SNPs（Single Nucleotide Polymorphism）detection and correlation study of multiparous traits were carried out on the double-lamb group of Tan sheep,aiming at promoting the application of SNPs in the genetic breeding of double-lamb in China. On the basis of SNPs screening at the previous studies,13 SNPs sites of 3 genes were determined as candidate sites by the flight mass spectrometry of candidate genes. Population differences were analyzed and multiparous candidate genes were screened by detecting polymorphism of the double-lamb group of Tan sheep. The association analyses showed that 5 of 13 loci were associated with multiparous performance,3 genetic models were confirmed,whereas haplotype constructed by BMPR1B gene was not associated with multiparous performance. The study is aimed at providing reference for molecule-assisted breeding techniques,such as molecule-marker species selection and breed selection for double-lamp group of Tan sheep in the protection and also a sustainable utilization of rare germplasm genetic resources of indigenous domestic animal,therefore accelerating the breeding progress of high quality multiparous mutton sheep.

Key words: Tan sheep, double-lamb, SNaPshot, prolificacy

TIAN De-hong, LIU Si-jia, DING Ning, LI Xue, ZHAO Kai. Research on SNP Associated with Multiparous Trait in Double-lamb of Tan Sheep by SNaPshot Assay[J]. Biotechnology Bulletin, 2019, 35(10): 152-161.

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