Comparative Study on Endophytic Fungi Diversity of Kobresia humilis in Floccularia luteovirens

doi:10.13560/j.cnki.biotech.bull.1985.2019-0385

Abstract

Abstract: In order to study the endophytic fungi diversity of Kobresia humilis in the habitat of Floccularia luteovirens,the diversity of endophytic fungi in K. humilis was studied by traditional isolation culture method and macrogenomic method. The results showed that：66 endophytic fungi were isolated from K. humilis and identified by traditional method,which belonged to Ascomycota and were classified into 7 orders,namely Pleosporales,Xylariales,Eurotiales,Helotiales and Hypocreales,Mucorales,Glomerellales. The dominant species were Stagonospora bicolor;the diversity index,richness index and evenness index of roots were 1.63,1.56 and 0.82,respectively. F. luteovirens was not obtained by isolation. 587 endophytic fungi were detected by macrogenomic method. Ascomycota accounted for 77.21%,Basidiomycota 16.21%,and the remaining 6.58% were unclassified fungi,the dominant population was Fusarium sp. The diversity index,richness index and evenness index of roots were 3.13,39.08 and 0.48,respectively. The F. luteovirens was detected and its relative abundance was 11.39. Comparing the two methods,there were abundant endophytic fungi resources in K. humilis in the habitat of F. luteovirens. F. luteovirens was one of the dominant endophytic fungi populations. This study proved for the first time that F. luteovirens was an endophytic fungus of Kobresia genus,it is a new breakthrough in studying the nutritional types of floccularia luteovirens and its interaction with plants.

Key words: Kobresia humilis, diversity of endophytic fungi, Floccularia luteovirens, traditional incubation method, metagenome method

GUO Jing, XIE Zhan-ling, LUO Tao, XUE Zhi-feng, GUO Jian-juan, LI Fa-xiong, ZHANG Xiu-juan. Comparative Study on Endophytic Fungi Diversity of Kobresia humilis in Floccularia luteovirens[J]. Biotechnology Bulletin, 2019, 35(11): 109-117.

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