Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (5): 64-67.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0701

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Qualitative PCR Assay for the Detection of GH5112E-117C Transgenic Maize Resistant to Insects and Herbicides

LI Cong-cong1, Xie Ping1, DONG Li-ming1, XIA Wei1, LAN Qing-kuo2, YAN Wei1, LONG Li-kun1, LI Fei-wu1   

  1. 1. Institute of Agricultural Quality Standard and Testing Technology,Jilin Academy of Agricultural Sciences,Changchun 130033;
    2. Tianjin Institute of Agricultural Quality Standard and Testing Technology,Tianjin 300381
  • Received:2019-08-06 Online:2020-05-26 Published:2020-06-03

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Abstract: Transgenic maize GH5112E-117C is a new strain with mG2-aroA gene and mcry1Ah genes and developed by Beijing Origen Company. It is resistant to lepidopteran pests such as corn borer and armyworm,and it is tolerant to glyphosate- herbicide. Thus this strain has important industrial application prospects in China. A qualitative PCR assay for transgenic maize GH5112E-117C was developed in this paper. The assay had promising specificity to detect the transgenic maize GH5112E-117C with resistance to insects and tolerance to herbicide. At a concentration of 0.2 μmol/L primer,TaqDNA polymerase 0.625 U,DNA template 50 ng,and an annealing temperature of 58℃,the detection sensitivity of the assay stably achieved to 0.1%. This assay provides a new technical means for accurately testing insect- and herbicide-resistant transgenic maize GH5112E-117C,which provides the technical support for transgenic supervision in agriculture.

Key words: transgenic maize, transformation event, PCR, detection assay