Isolation and Identification of the Growth-promoting Bacteria from Pbycosphere of Dunaliella salina

doi:10.13560/j.cnki.biotech.bull.1985.2019-1264

Abstract

Abstract: Through exploring the influence of phycosphere microorganisms on the microalgae growth and metabolite accumulation,the predominant growth-promoting strains were screened. The symbiotic bacterial strains from the phycosphere of Dunaliella salina Ds-SXYC-2 were isolated and identified,and 1∶1 algal-bacteria co-culture system was constructed,and the growth of D. salina and the accumulation of its metabolite were measured. The results showed that 5 symbiotic bacterial strains were isolated from the phycosphere of D. salina,and they belonged to 3 genera,including Nesterenkonia（Strain B1 and B2）,Halomonas（strain B3 and B4）,and Marinobacter（Strain B5）. All the 5 strains promoted the growth of D. salina. Among them,strain B3 significantly promoted the growth and the accumulation of metabolites. After 15 d of co-culture,the biomass of D. salina reached 2.3 g/L,which was higher than that of the control without bacteria and increased by 28.9%. The content of chlorophyll a（4.61 mg/L）and beta-carotene increased by 36.3% and 56.4%,respectively,in contrast to the control. The contents of intracellular polysaccharide,proteins and total lipids increased by 34.8%,71.2% and 37.6%,respectively,compared with the control group. Halomonas strain B3 could be used as the dominant strain to promote the growth and metabolite accumulation of D. salina,and the co-culture system could be constructed and applied to the commercial production of D. salina.

Key words: Dunaliella salina, bacterial flora in phycosphere, algal-bacteria co-culture system

DUAN Lu-lu, HANG Wei, CHENG Yu-jiao, CUI Hong-li, WANG Ji-ping, LI Run-zhi. Isolation and Identification of the Growth-promoting Bacteria from Pbycosphere of Dunaliella salina[J]. Biotechnology Bulletin, 2020, 36(5): 169-175.

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