Enhanced Furfural Tolerance in Saccharomyces cerevisiae by the Overexpression of GLN1 Gene

doi:10.13560/j.cnki.biotech.bull.1985.2020-0033

Abstract

Abstract: Furfural is the main inhibitor of straw hydrolysate,and it can inhibit the growth and fermentation performance of Saccharomyces cerevisiae. The glutamine synthetase gene GLN1 of S. cerevisiae was inserted into the vector pESC-URA,and then introduced into the original yeast BY4741 to construct the recombinant yeast YEA9,and its furfural tolerance and fermentation performance were measured. The results showed that at a concentration of 1.55 g / L furfural,the biomass of YEA9 increased by 3.7 times,the sugar consumption rate increased by 8.6 times,the final ethanol concentration increased by 9.2 times,and the sugar alcohol conversion rate increased by 10.6%. Glutathione content increased by 22.4%,NADH oxidase activity increased by 5.1%,NAD ⁺ / NADH values did not change significantly,and the level of reactive oxygen species was lower than that in BY4741. In the simulated hydrolysate,the biomass of YEA9 increased by 1.75 times,the sugar consumption rate increased by 6.1 times,the final ethanol content increased by 5.3 times,and the sugar alcohol conversion rate increased by 0.5%. In summary,overexpression of the GLN1 gene can increase the enzyme activity of glutamine synthetase in S. cerevisiae,the content of glutamine and glutathione,and the enzyme activity of NADH oxidase,while reduce the accumulation of ROS,and ultimately enhance the furfural stress tolerance and ethanol fermentation efficiency.

Key words: furfural, Saccharomyces cerevisiae, straw hydrolysate, fiber ethanol, fermentation

WU Yu, WANG Jin-hua, ZHAO Xiao. Enhanced Furfural Tolerance in Saccharomyces cerevisiae by the Overexpression of GLN1 Gene[J]. Biotechnology Bulletin, 2020, 36(8): 69-78.

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