Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (10): 88-98.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0097

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Secondary Metabolic Pathway Mining and Pan-genome Analysis of Bacillus coagulans

QIAN Wen-jiang1,2(), WANG Bu-qing1,2, LI Wei-xi1,2, YANG Xue-miao2, LIU Hong-wei2(), ZHANG Li-ping1,2()   

  1. 1. Hebei University of Technology,Tianjin 300130
    2. Institute of Biology,Hebei Academy of Science,Shijiazhuang 050081
  • Received:2020-02-02 Online:2020-10-26 Published:2020-11-02
  • Contact: LIU Hong-wei,ZHANG Li-ping E-mail:qianweijiang2020@163.com;lhwei1987@126.com;wsw4879@163.com

Abstract:

This study was to further understand the genome of Bacillus coagulans and to explore its secondary metabolic pathways. The genomes of 33 B. coagulans were downloaded from the NCBI database and analyzed by pan-genomic analysis software and secondary metabolite mining software. The genome size of 33 strains of B. coagulans was between 2.059 47-3.694 84 Mbp and the GC content ranged from 46.2% to 47.5%. Pan-genome analysis of 11 strains of B. coagulans with complete genome level revealed that the pan-genome contained 5 899 cluster genes,the core genome consisted of 2 152 cluster genes,and 2 255 strain-specific genes were identified. Based on the analysis of secondary metabolite synthesis gene clusters,79 secondary metabolite gene clusters of 8 groups were found in the 33 B. coagulans genomes. The major synthetic gene clusters were bacteriocins,lactones and sugars. In brief,the pan-genome and core genome sizes of B. coagulans and their secondary metabolites through can be predicted in this study,which is conducive to understanding B. coagulans and providing relevant information further better utilization of this strain.

Key words: Bacillus coagulans, pan-genome, secondary metabolic gene cluster