Analysis of Mutation Sites of OsBADH2 Gene in Fragrant Rice and Development of Related Functional Marker

doi:10.13560/j.cnki.biotech.bull.1985.2020-1086

Abstract

Abstract:

Fragrant rice is favored worldwide by consumers and rice breeders,due to its full aroma and high nutritional value. Exploring and utilizing the flagrant gene resources would lay a foundation for breeding excellent-quality varieties. Loss of function of the betaine aldehyde dehydrogenase gene（OsBADH2）leads to that rice is of fragrance. OsBADH2 coding regions in 10 fragrant rice varieties were sequenced to explicit its mutant type. A functional marker E7 was designed according to the 8-bp deletion in exon 7 of OsBADH2 in the fragrant rice. Genotypes of 10 fragrant rice varieties and 2 populations derived from fragrant rice/non-fragrant varieties were identified and verified by E7. The results indicated that 8 bp deletion and 3 bp replacement in the exon 7 of OsBADH2 gene were found in 9 cultivars,including Xiangyaxiangzhan,Huaxiang B and Nongxiang 24 et a1. And 1 bp（G）insertion mutation in exon 14 in the fragrant rice of Xiao7 appeared. The homozygous dominant,homozygous recessive and heterozygous genotypes of OsBADH2 could be rapidly and accurately distinguished by functional marker E7.

Key words: fragrant rice, OsBADH2, functional marker, molecular marker-assisted selection

SUN Ping-yong, ZHANG Wu-han, SHU Fu, HE Qiang, ZHANG Li, PENG Zhi-rong, DENG Hua-feng. Analysis of Mutation Sites of OsBADH2 Gene in Fragrant Rice and Development of Related Functional Marker[J]. Biotechnology Bulletin, 2021, 37(4): 1-7.

Figures/Tables 6

Fig. 1 Amplification result from coding regions of OsBADH2

Fig. 2 Genetic structure of OsBADH2 and allelic variation types in fragrant rice

Fig.3 Location of functional marker E7 in the OsBADH2

Fig. 4 Molecular detection of OsBADH2 genotypes by functional marker E7 in different rice varieties M: 100 bp marker. 1-12: Basmati, Xiangza 1, Yuyouxiangzhan, Xiangyaxiangzhan, Huaxiang B, Nongxiang 24, Daohuaxiang, Xianggeng, Mingxing10S, xiao7, Mowanggu, and 02428

Fig. 5 Content of 2AP in different rice varieties a, b and c represent significant differences

Fig. 6 Molecular detection of OsBADH2 genotypes by functional marker E7 in two breeding populations M: 100 bp marker. (A) 1: Daohuaxiang. 2: Geng 944, 3-30: Parts of plants from Geng 944 / Daohuaxiang//Geng 944 population. (B) 1: Mingxing10S, 2: Taifeng B. 3-30: Parts of plants from Taifeng B/Mingxing10S // Taifeng B

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